Liu Hualei, Wang Zhiliang, Son Cuiping, Wang Yongkun, Yu Bin, Zheng Dongxia, Sun Chengying, Wu Yangong
National Reference Laboratory for Newcastle Disease, Animal Quarantine Institute, Ministry of Agriculture, Qingdao, China 260032.
Avian Dis. 2006 Dec;50(4):636-40. doi: 10.1637/7618-042606R1.1.
Fourteen pigeon-origin Newcastle disease virus (NDV) isolates were obtained from sick pigeons in China between 1996 and 2005. The mean death time (MDT) of embryonated eggs and the intracerebral pathogenicity indices (ICPI) were tested to determine the virulence of the field isolates. The result indicated that most isolates were proved to be mesogenic (MDT 60-90 hr and ICPI > 1.2). The main function regions of F protein gene of the isolates were amplified and sequenced for phylogenetic and residue substitutive analysis. The fusion protein cleavage site sequences of most isolates had multiple basic amino acids R/KRQKRF at positions 112-116 and a phenyl alanine at position 117, characteristic of velogenic isolates. In the phylogenetic tree, the majority of the isolates were clustered into a single genetic lineage, termed genotype VIb, and were typical pigeon paramyxovirus type 1, whereas a small number of recent isolates (three strains) were grouped into genotype VIId, a predominant genotype responsible for most Newcastle disease outbreaks in chickens and geese since the end of last century. One isolate, PK9901, was proved to be a lentogenic strain, of genotype II NDV, to which the vaccine strain La Sota belongs.
1996年至2005年间，从中国患病鸽子中分离出14株鸽源新城疫病毒（NDV）。通过检测鸡胚平均死亡时间（MDT）和脑内致病指数（ICPI）来确定这些田间分离株的毒力。结果表明，大多数分离株被证明为中等毒力（MDT为60 - 90小时，ICPI > 1.2）。对分离株F蛋白基因的主要功能区进行扩增和测序，以进行系统发育和氨基酸替代分析。大多数分离株的融合蛋白裂解位点序列在112 - 116位有多个碱性氨基酸R/KRQKRF，在117位有一个苯丙氨酸，这是强毒株的特征。在系统发育树中，大多数分离株聚为一个单一的遗传谱系，称为基因型VIb，是典型的1型鸽副粘病毒，而少数近期分离株（三个毒株）被归入基因型VIId，这是自上世纪末以来导致大多数鸡和鹅新城疫爆发的主要基因型。一个分离株PK9901被证明是弱毒株，属于疫苗株La Sota所属的基因型II NDV。
