Barbezange Cyril, Jestin Véronique
AFSSA (French Agency for Food Safety), Avian and Rabbit Virology Immunology and Parasitology Unit, BP 53, 22440 Ploufragan, France.
Virus Genes. 2003;26(2):175-83. doi: 10.1023/a:1023439530750.
Three avian Paramyxovirus type 1 (aPMV-1) isolated from pigeons duringpigeon paramyxovirosis outbreaks were molecularly characterised by sequencing parts of the six genes (NP, P, M, F, HN and L) of each strain. Virulent 99143 isolate was found to be very closely related to non-pathogenic vaccine strains of aPMV-1, even for its F protein cleavage site motif. Strains 99299 and 99106, typical pigeon paramyxovirus type 1 (pPMV-1) variants, exhibited between 10% and 20% difference with aPMV-1 at the nucleotide level. The aPMV-1 specific pattern of eight amino acids in the intracellular domain of HN protein was found different by one residue for these two isolates, and might represent a specific pattern for pPMV-1. The unique sequence of the polycistronic P gene editing site of 99299 and 99106 was characterised by four instead of three cytosine residues, and might so have an influence on the expression level of the three proteins encoded by P. This work is also the first to provide molecular data on NP, P and L genes of typical pPMV-1.
在鸽副粘病毒病暴发期间，从鸽子中分离出三株禽1型副粘病毒（aPMV-1），通过对每个毒株的六个基因（NP、P、M、F、HN和L）的部分序列进行测序，对其进行了分子特征分析。发现强毒株99143即使在其F蛋白裂解位点基序方面，也与aPMV-1的非致病性疫苗毒株密切相关。99299和99106毒株是典型的鸽1型副粘病毒（pPMV-1）变体，在核苷酸水平上与aPMV-1有10%至20%的差异。发现这两个分离株的HN蛋白胞内结构域中八个氨基酸的aPMV-1特异性模式有一个残基不同，这可能代表pPMV-1的一种特异性模式。99299和99106的多顺反子P基因编辑位点的独特序列由四个而非三个胞嘧啶残基组成，因此可能会影响P基因编码的三种蛋白质的表达水平。这项工作也是首次提供典型pPMV-1的NP、P和L基因的分子数据。
