Zaatreh Sarah, Haffner David, Strauß Madlen, Wegner Katharina, Warkentin Mareike, Lurtz Claudia, Zamponi Christiane, Mittelmeier Wolfram, Kreikemeyer Bernd, Willumeit-Römer Regine, Quandt Eckhard, Bader Rainer
a Biomechanics and Implant Technology Research Laboratory, Department of Orthopedics , University Medicine Rostock , Rostock , Germany.
b Inorganic Functional Materials, Institute of Materials Science, Faculty of Engineering , Kiel University , Kiel , Germany.
Biofouling. 2017 Apr;33(4):294-305. doi: 10.1080/08927014.2017.1303832. Epub 2017 Mar 28.
Bacterial colonisation and biofilm formation are characteristics of implant-associated infections. In search of candidates for improved prosthetic materials, fast corroding Mg-based coatings on titanium surfaces were examined for their cytotoxic and antimicrobial properties. Human osteoblasts and Staphylococcus epidermidis were each cultured on cylindrical Ti samples coated with a thin layer of Mg/MgZnCa, applied via magnetron sputtering. Uncoated titanium samples served as controls. S. epidermidis was quantified by counting colony forming units. The biofilm-bound fraction was isolated via ultrasonic treatment, and the planktonic fraction via centrifugation. Biofilm-bound S. epidermidis was significantly decreased by approximately four to five orders of magnitude in both Mg- and MgZnCa-coated samples after seven days compared to the control. The osteoblast viability was within the tolerance threshold of 70% stated in DIN EN ISO 10993-5:2009-10 for Mg (80%) but not for MgZnCa (25%). Accordingly, Mg-coated titanium was identified as a promising candidate for an implant material with antibacterial properties and low cytotoxicity levels. The approach of exploiting fast corrosion contrasts with existing methods, which have generally focused on reducing corrosion.
细菌定植和生物膜形成是植入物相关感染的特征。为了寻找性能更优的假体材料候选物，研究了钛表面快速腐蚀的镁基涂层的细胞毒性和抗菌性能。通过磁控溅射在圆柱形钛样品上涂覆一层Mg/MgZnCa薄层，分别培养人成骨细胞和表皮葡萄球菌。未涂覆的钛样品用作对照。通过计数菌落形成单位对表皮葡萄球菌进行定量。通过超声处理分离生物膜结合部分，通过离心分离浮游部分。与对照相比，七天后，Mg涂层和MgZnCa涂层样品中生物膜结合的表皮葡萄球菌均显著减少了约四到五个数量级。成骨细胞活力在DIN EN ISO 10993-5:2009-10规定的Mg（约80%）的70%耐受阈值内，但MgZnCa（约25%）不在此范围内。因此，Mg涂层钛被确定为具有抗菌性能和低细胞毒性水平的植入材料的有前途的候选物。利用快速腐蚀的方法与现有方法形成对比，现有方法通常侧重于减少腐蚀。
