Department of Periodontology, Center of Dental and Oral Medicine, University Hospital, Bonn, Germany.
Department of Physics, Freie Universität Berlin, Berlin, Germany; Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
Nanomedicine. 2017 May;13(4):1587-1593. doi: 10.1016/j.nano.2017.01.002. Epub 2017 Jan 20.
Bacterial biofilms cause a considerable amount of prosthetic joint infections every year, resulting in morbidity and expensive revision surgery. To address this problem, surface modifications of implant materials such as carbon nanotube (CNT) coatings have been investigated in the past years. CNTs are biologically compatible and can be utilized as drug delivery systems. In this study, multi-walled carbon nanotube (MWCNT) coated TiAl6V4 titanium alloy discs were fabricated and impregnated with Rifampicin, and tested for their ability to prevent biofilm formation over a period of ten days. Agar plate-based assays were employed to assess the antimicrobial activity of these surfaces against Staphylococcus epidermidis. It was shown that vertically aligned MWCNTs were more stable against attrition on rough surfaces than on polished TiAl6V4 surfaces. Discs with coated surfaces caused a significant inhibition of biofilm formation for up to five days. Therefore, MWCNT-modified surfaces may be effective against pathogenic biofilm formation on endoprostheses.
细菌生物膜每年都会导致大量的人工关节感染，造成发病率上升和昂贵的翻修手术。为了解决这个问题，过去几年一直在研究植入材料的表面改性，如碳纳米管（CNT）涂层。CNTs 具有生物相容性，可以用作药物输送系统。在这项研究中，制备了多壁碳纳米管（MWCNT）涂覆的 TiAl6V4 钛合金圆盘，并将利福平浸渍在其中，以测试其在十天内防止生物膜形成的能力。采用琼脂平板法评估这些表面对表皮葡萄球菌的抗菌活性。结果表明，垂直排列的 MWCNTs 在粗糙表面上比在抛光 TiAl6V4 表面上更稳定，不易磨损。涂覆表面的圆盘在长达五天的时间内显著抑制了生物膜的形成。因此，MWCNT 改性表面可能对抑制内植物上的致病生物膜形成有效。
