Division of Gastroenterology, Department of Pediatrics, BC Children's Hospital and the University of British Columbia, Vancouver, BC, Canada.
Nutrition and Metabolism Research Program, Department of Pediatrics, BC Children's Hospital and the University of British Columbia, Vancouver, BC, Canada.
Sci Rep. 2017 Mar 28;7:45274. doi: 10.1038/srep45274.
Breast milk has many beneficial properties and unusual characteristics including a unique fat component, termed milk fat globule membrane (MFGM). While breast milk yields important developmental benefits, there are situations where it is unavailable resulting in a need for formula feeding. Most formulas do not contain MFGM, but derive their lipids from vegetable sources, which differ greatly in size and composition. Here we tested the effects of MFGM supplementation on intestinal development and the microbiome as well as its potential to protect against Clostridium difficile induced colitis. The pup-in-a-cup model was used to deliver either control or MFGM supplemented formula to rats from 5 to 15 days of age; with mother's milk (MM) reared animals used as controls. While CTL formula yielded significant deficits in intestinal development as compared to MM littermates, addition of MFGM to formula restored intestinal growth, Paneth and goblet cell numbers, and tight junction protein patterns to that of MM pups. Moreover, the gut microbiota of MFGM and MM pups displayed greater similarities than CTL, and proved protective against C. difficile toxin induced inflammation. Our study thus demonstrates that addition of MFGM to formula promotes development of the intestinal epithelium and microbiome and protects against inflammation.
母乳具有许多有益的特性和独特的特征，包括一种独特的脂肪成分，称为乳脂肪球膜 (MFGM)。虽然母乳能带来重要的发育益处，但有时会因为无法获得母乳而需要配方奶喂养。大多数配方奶不含 MFGM，而是从植物来源中获取脂质，而植物来源的脂质在大小和组成上有很大的不同。在这里，我们测试了 MFGM 补充对肠道发育和微生物组的影响，以及它预防艰难梭菌诱导的结肠炎的潜力。使用杯中之鼠模型从 5 到 15 天大的大鼠中给予对照或 MFGM 补充配方；用母乳喂养的动物作为对照。虽然与 MM 同窝仔相比，CTL 配方显著降低了肠道发育，但在配方中添加 MFGM 可使肠道生长、潘氏细胞和杯状细胞数量以及紧密连接蛋白模式恢复到 MM 仔鼠的水平。此外，MFGM 和 MM 仔鼠的肠道微生物组与 CTL 相比具有更大的相似性，并能预防艰难梭菌毒素诱导的炎症。因此，我们的研究表明，在配方中添加 MFGM 可促进肠道上皮和微生物组的发育，并预防炎症。
