Nan Wenbin, Xu Zhihao, Chen Zhibin, Yuan Xin, Lin Juntang, Feng Huigen, Lian Jie, Chen Hongli
Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
Research Center of Stem Cell and Biotherapy Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
Mol Med Rep. 2017 May;15(5):3239-3244. doi: 10.3892/mmr.2017.6400. Epub 2017 Mar 28.
The aim of the present study was to evaluate the healing effect of bone marrow-derived mesenchymal stem cells administered to hyperglycemia model mice with skin wounds, and to explore the underlying mechanism contributing to their effects in promoting refractory wound healing. A full‑thickness skin wound mouse model was established, and refers to a wound of the skin and subcutaneous tissue. The mice were randomly divided into three groups: Blank control group, hyperglycemic group and a hyperglycemic group treated with stem cells. Wound healing was monitored and the wound‑healing rate was determined at 3, 6, 9, and 12 days following trauma. The structure of the organization of new skin tissue was observed by hematoxylin and eosin staining, and expression levels of the inflammatory cytokines interleukin (IL)‑6 and tumor necrosis factor (TNF)‑α were determined from 1 to 6 days following trauma. The wound healing of the hyperglycemic group was slower than that of the blank group, and the hyperglycemic mice treated with stem cells presented faster healing than the hyperglycemia group. The horny layer and granular layer of the skin were thinner and incomplete in the new skin tissue of the hyperglycemic group, whereas the new skin wound tissue basal layer was flat and demonstrated better fusion with the wound edge in the other two groups. The expression of inflammatory cytokines (IL‑6 and TNF‑α) was significantly increased in all three groups, with continuously higher expression in the hyperglycemic group and decreased expression in the other two groups over time. Hyperglycemia refractory wounds are likely related to the excessive expression of inflammatory cytokines surrounding the wound area. Stem cells may be able to alleviate the excessive inflammatory reaction in the wound tissue of hyperglycemic mice, so as to promote wound healing.
本研究的目的是评估给予皮肤创伤的高血糖模型小鼠骨髓间充质干细胞的愈合效果，并探讨其促进难治性伤口愈合作用的潜在机制。建立了全层皮肤创伤小鼠模型，该模型指皮肤和皮下组织的创伤。将小鼠随机分为三组：空白对照组、高血糖组和干细胞治疗的高血糖组。在创伤后3、6、9和12天监测伤口愈合情况并测定伤口愈合率。通过苏木精和伊红染色观察新皮肤组织的组织结构，并在创伤后1至6天测定炎性细胞因子白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α的表达水平。高血糖组的伤口愈合比空白组慢，而接受干细胞治疗的高血糖小鼠的愈合速度比高血糖组快。高血糖组新皮肤组织中皮肤角质层和颗粒层较薄且不完整，而其他两组新皮肤伤口组织基底层平坦，与伤口边缘融合较好。所有三组中炎性细胞因子（IL-6和TNF-α）的表达均显著增加，高血糖组随时间持续表达更高，而其他两组表达下降。高血糖难治性伤口可能与伤口周围炎性细胞因子的过度表达有关。干细胞可能能够减轻高血糖小鼠伤口组织中的过度炎症反应，从而促进伤口愈合。
