Gong Chen, Xia Chengde, Liu Linbo
Department of Plastic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Department of Burn Surgery, The First People's Hospital of Zhengzhou, Zhengzhou, Henan, China.
Regen Ther. 2024 Oct 23;26:932-940. doi: 10.1016/j.reth.2024.09.009. eCollection 2024 Jun.
Diabetic wounds are difficult to repair effectively in the clinic. Tissue engineering based on mesenchymal stem cells (MSCs) showed great therapeutic potential in wound healing. MSCs-derived exosome could reproduce the effect of MSCs by transferring the bioactive substance to the recipient cells. The biological function of exosomes was determined by the state of the derived MSCs. In this study, we cultured hUC-MSCs with EGFL6 and isolated EGFL6-preconditioned exosomes (EGF-Exos), and then investigated the effect of EGF-Exos on wound healing. The results revealed that EGF-Exos promoted the proliferation and migration of HUVECs, had the anti-inflammtory function and improved angiogenesis. Moreover, we fabricated Gelama hydrogel to load EGF-Exos to repair diabetic wounds. results showed that EGF-Exos contributed to the repair of diabetic wound and provided valuable data for understanding the role of EGF-Exos in diabetic wound healing.
糖尿病伤口在临床上难以有效修复。基于间充质干细胞（MSCs）的组织工程在伤口愈合方面显示出巨大的治疗潜力。MSCs来源的外泌体可通过将生物活性物质转移至受体细胞而重现MSCs的作用。外泌体的生物学功能由其来源的MSCs状态决定。在本研究中，我们用EGFL6培养人脐带来源的间充质干细胞（hUC-MSCs）并分离出经EGFL6预处理的外泌体（EGF-Exos），然后研究EGF-Exos对伤口愈合的影响。结果显示，EGF-Exos促进了人脐静脉内皮细胞（HUVECs）的增殖和迁移，具有抗炎功能并改善了血管生成。此外，我们制备了Gelama水凝胶来负载EGF-Exos以修复糖尿病伤口。结果表明，EGF-Exos有助于糖尿病伤口的修复，并为理解EGF-Exos在糖尿病伤口愈合中的作用提供了有价值的数据。
