Ionita Radu, Postu Paula Alexandra, Beppe Galba Jean, Mihasan Marius, Petre Brindusa Alina, Hancianu Monica, Cioanca Oana, Hritcu Lucian
Department of Biology, Alexandru Ioan Cuza University of Iasi, Bd. Carol I, No. 11, 700506, Iasi, Romania.
Laboratory of Animal Physiology, Faculty of Science, University of Yaoundé I, PO Box, 812, Yaoundé, Cameroon.
Behav Brain Funct. 2017 Mar 28;13(1):5. doi: 10.1186/s12993-017-0123-6.
Plants of the genus Markhamia have been traditionally used by different tribes in various parts of West African countries, including Cameroun. Markhamia tomentosa (Benth.) K. Schum. (Bignoniaceae) is used as an antimalarial, anti-inflammatory, analgesic, antioxidant and anti-Alzheimer agent. The current study was undertaken in order to investigate its anti-amnesic and antioxidant potential on scopolamine-induced cognitive impairment and to determine its possible mechanism of action.
Rats were pretreated with the aqueous extract (50 and 200 mg/kg, p.o.), for 10 days, and received a single injection of scopolamine (0.7 mg/kg, i.p.) before training in Y-maze and radial arm-maze tests. The biochemical parameters in the rat hippocampus were also assessed to explore oxidative status. Statistical analyses were performed using two-way ANOVA followed by Tukey's post hoc test. F values for which p < 0.05 were regarded as statistically significant.
In the scopolamine-treated rats, the aqueous extract improved memory in behavioral tests and decreased the oxidative stress in the rat hippocampus. Also, the aqueous extract exhibited anti-acetylcholinesterase activity.
These results suggest that the aqueous extract ameliorates scopolamine-induced spatial memory impairment by attenuation of the oxidative stress in the rat hippocampus.
马克汉木属植物在包括喀麦隆在内的西非国家不同地区的部落中一直有传统用途。绒毛马克汉木（Markhamia tomentosa (Benth.) K. Schum.，紫葳科）被用作抗疟疾、抗炎、止痛、抗氧化和抗阿尔茨海默病药物。开展本研究是为了调查其对东莨菪碱诱导的认知障碍的抗遗忘和抗氧化潜力，并确定其可能的作用机制。
大鼠用该水提取物（50和200毫克/千克，口服）预处理10天，并在Y迷宫和放射状臂迷宫试验训练前单次注射东莨菪碱（0.7毫克/千克，腹腔注射）。还评估了大鼠海马体中的生化参数以探索氧化状态。使用双向方差分析，随后进行Tukey事后检验进行统计分析。p < 0.05的F值被视为具有统计学意义。
在经东莨菪碱处理的大鼠中，水提取物改善了行为测试中的记忆力，并降低了大鼠海马体中的氧化应激。此外，水提取物表现出抗乙酰胆碱酯酶活性。
这些结果表明，水提取物通过减轻大鼠海马体中的氧化应激来改善东莨菪碱诱导的空间记忆障碍。
