Ozarowski Marcin, Mikolajczak Przemyslaw L, Piasecka Anna, Kujawski Radoslaw, Bartkowiak-Wieczorek Joanna, Bogacz Anna, Szulc Michal, Kaminska Ewa, Kujawska Malgorzata, Gryszczynska Agnieszka, Kachlicki Piotr, Buchwald Waldemar, Klejewski Andrzej, Seremak-Mrozikiewicz Agnieszka
Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, Sw. Marii Magdaleny 14, Poznan, Poland; Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland.
Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Wojska Polskiego 71b, 60-630 Poznan, Poland; Department of Pharmacology, University of Medical Sciences, Rokietnicka 5a, 60-806 Poznan, Poland.
Physiol Behav. 2017 May 1;173:223-230. doi: 10.1016/j.physbeh.2017.02.019. Epub 2017 Feb 17.
Salvia miltiorrhiza (Lamiaceae), one of the most important and popular plants of traditional medicine of Asia, is used for the prevention and treatment of cardiovascular diseases and in central nervous system disturbances. The main aim of this study was to assess the influence of subchronic (28-fold) administration of Salvia miltiorrhiza root extract (SE, 200mg/kg, p.o.) on behavioural activity and memory of rats and to evaluate the activities of cholinesterases (AChE and BuChE) and gene expression levels of AChE and BuChE as well as of beta-secretase (BACE1) in the hippocampus and frontal cortex in vivo. Huperzine A (HU, 0.5mg/kg b.w., p.o.) served as a positive control substance, whereas scopolamine (0.5mg/kg, i.p.) injection was used as a well-known model of memory impairment. The results showed that subchronic administration of SE led to an improvement of long-term memory of rats. Strong inhibition of AChE and BuChE mRNA transcription in the frontal cortex of rats treated with SE or HU was observed. The BACE1 transcript level was significantly decreased. AChE activity was statistically significantly inhibited in the frontal cortex and the hippocampus by SE (47% and 55%, respectively). Similar effects were observed in the case of HU. In summary, activity of SE provides evidence that the plant can be a source of drugs used in the treatment of Alzheimer disease.
丹参（唇形科）是亚洲传统医学中最重要且最常用的植物之一，用于预防和治疗心血管疾病以及中枢神经系统紊乱。本研究的主要目的是评估亚慢性（28天）给予丹参根提取物（SE，200mg/kg，口服）对大鼠行为活动和记忆的影响，并在体内评估海马体和额叶皮质中胆碱酯酶（AChE和BuChE）的活性以及AChE、BuChE和β-分泌酶（BACE1）的基因表达水平。石杉碱甲（HU，0.5mg/kg体重，口服）作为阳性对照物质，而注射东莨菪碱（0.5mg/kg，腹腔注射）作为众所周知的记忆损伤模型。结果表明，亚慢性给予SE可改善大鼠的长期记忆。观察到用SE或HU处理的大鼠额叶皮质中AChE和BuChE mRNA转录受到强烈抑制。BACE1转录水平显著降低。SE在额叶皮质和海马体中对AChE活性有统计学显著抑制作用（分别为47%和55%）。HU也观察到类似效果。总之，SE的活性证明该植物可作为治疗阿尔茨海默病药物的来源。
