Université Clermont Auvergne, Université Blaise Pascal, Institut Pascal, BP 10448, F-63000 Clermont-Ferrand, France; CNRS - UMR 6602, IP, F-63178 Aubière, France; Lebanese University, AZM Centre for Research in Biotechnology and Its Applications, Laboratory of Applied Biotechnology for Biomolecules, Biotherapy and Bioprocess, El Mitein Street, Tripoli, Lebanon.
Université Clermont Auvergne, Université Blaise Pascal, Institut Pascal, BP 10448, F-63000 Clermont-Ferrand, France; CNRS - UMR 6602, IP, F-63178 Aubière, France.
Bioresour Technol. 2017 Jul;235:25-34. doi: 10.1016/j.biortech.2017.03.056. Epub 2017 Mar 11.
The aim of this work was to develop a strategy for second-generation ethanol production from carob solid waste issued from Lebanese food industry. The pros and cons of submerged (SF) and solid-state fermentations (SSF) using S. cerevisiae on ethanol yield and productivity were compared, including the respective roles of upstream and downstream processes, such as the size reduction, or sugar and ethanol recovery processes. The design of experiments methodology was applied. Experimental results demonstrated that SSF applied to cut carob waste from carob syrup preparation was simpler to operate and more cost-effective, maintained yield and productivity (0.458g ethanol/g consumed sugar and 4.3g/(kg waste)/h) in comparison to SF (0.450g ethanol/g consumed sugar and 5.7g/(kg waste)/h), and was able to achieve ethanol production up to 155g/(kg waste) at low water demand, while SF reached only 78g/(kg waste) due to the limitations of the sugar extraction pretreatment.
本工作旨在开发一种从黎巴嫩食品工业产生的角豆固体废料中生产第二代乙醇的策略。比较了使用酿酒酵母进行浸没（SF）和固态发酵（SSF）在乙醇产量和生产率方面的优缺点，包括上游和下游过程的各自作用，例如减小尺寸，或糖和乙醇回收过程。应用了实验设计方法。实验结果表明，与 SF（0.450g 乙醇/g 消耗糖和 5.7g/（kg 废物）/h）相比，SSF 更适用于切割角豆糖浆制备过程中产生的角豆固体废料，操作更简单，更具成本效益，维持了产量和生产率（0.458g 乙醇/g 消耗糖和 4.3g/（kg 废物）/h），并且能够在低耗水量下达到 155g/（kg 废物）的乙醇产量，而 SF 仅达到 78g/（kg 废物），因为糖提取预处理存在限制。
