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两种柑橘接穗-砧木组合叶片中与硼缺乏相关的转录组变化

Transcriptome Changes Associated with Boron Deficiency in Leaves of Two Citrus Scion-Rootstock Combinations.

作者信息

Liu Xiao, Zhang Jia-Wei, Guo Ling-Xia, Liu Yong-Zhong, Jin Long-Fei, Hussain Syed Bilal, Du Wei, Deng Zhao, Peng Shu-Ang

机构信息

Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan, China.

出版信息

Front Plant Sci. 2017 Mar 14;8:317. doi: 10.3389/fpls.2017.00317. eCollection 2017.

DOI:10.3389/fpls.2017.00317
PMID:28352276
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5349144/
Abstract

Boron (B) deficiency stress is frequently observed in citrus orchards and causes considerable loss of productivity and fruit quality. Carrizo citrange (Cc) has been reported as a rootstock more tolerant to B deficiency than Trifoliate orange (To). The 'Newhall' navel orange (Ns) performed better when grafted onto Cc (Ns/Cc) than when grafted onto To (Ns/To) under long-term B deficiency. The present study confirmed that Ns/Cc had higher boron content, leaf fresh weight, lower leaf chlorosis and stronger photosynthesis ability than Ns/To. Moreover, B-deficiency significantly reduced the chlorophyll and carotenoid content in Ns/To. The content of total soluble sugar and lignin were dramatically increased and the expression levels of photosynthesis-related genes were substantially down-regulated in Ns/To by B-deficient treatment. B-deficiency also strongly induced expression levels of chlorophyll decomposition-related genes, glucose synthesis-related genes and lignin synthesis-related genes, and significantly inhibited the expression of carotenoid synthesis-related genes in Ns/To. Overall, these findings suggested that the influence of To on the scion of Ns was worse than that of Cc due to differently regulating these metabolic pathways under the long term of B-deficiency. The transcriptome analysis provided further information for understanding the mechanism of the different responses of scion-rootstock combinations to B-deficiency stress.

摘要

硼（B）缺乏胁迫在柑橘园中经常出现，会导致生产力和果实品质大幅下降。据报道，卡里佐枳橙（Cc）作为砧木比枳壳（To）更耐硼缺乏。在长期硼缺乏条件下，‘纽荷尔’脐橙（Ns）嫁接在Cc（Ns/Cc）上比嫁接在To（Ns/To）上表现更好。本研究证实，Ns/Cc比Ns/To具有更高的硼含量、叶片鲜重、更低的叶片黄化程度和更强的光合作用能力。此外，硼缺乏显著降低了Ns/To中的叶绿素和类胡萝卜素含量。硼缺乏处理使Ns/To中的总可溶性糖和木质素含量显著增加，光合作用相关基因的表达水平大幅下调。硼缺乏还强烈诱导了Ns/To中叶绿素分解相关基因、葡萄糖合成相关基因和木质素合成相关基因的表达水平，并显著抑制了类胡萝卜素合成相关基因的表达。总体而言，这些发现表明，由于在长期硼缺乏条件下对这些代谢途径的调控不同，To对Ns接穗的影响比Cc更差。转录组分析为理解接穗 - 砧木组合对硼缺乏胁迫不同反应的机制提供了进一步的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ccc/5349144/6f789810e9d3/fpls-08-00317-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ccc/5349144/6f789810e9d3/fpls-08-00317-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ccc/5349144/8a7693c27858/fpls-08-00317-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ccc/5349144/31cfd9e6c5f8/fpls-08-00317-g002.jpg
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