Remya R R, Rajasree S R Radhika, Aranganathan L, Suman T Y
Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, Tamilnadu, India.
Biotechnol Rep (Amst). 2015 Oct 22;8:110-115. doi: 10.1016/j.btre.2015.10.004. eCollection 2015 Dec.
A single step protocol to produce biofunctionalized silver nanoparticles (AgNPs) using the aqueous extract of flower as "natural factory" was investigated. The reaction between silver ions and aqueous flower extract after the bioreduction process has resulted in the formation of reddish brown color colloidal solution. XRD pattern showed the face centered cubic crystalline structure of AgNPs and exhibited spherical morphology as characterized by FE-SEM. FTIR studies identified different functional groups involved in effective capping of AgNPs. The affirmed the phytoreduced AgNPs possess good stability and the size of the particle was measured by DLS. The synthesized AgNPs displayed effective cytotoxic potential against MCF7 and the inhibitory concentration (IC) was recorded at 7.19 μg/mL. The apoptotic effects of the AgNPs were also confirmed by AO/EB staining. The investigation presents preliminary evidence that biosynthesized AgNPs can be used in the development of novel anticancer drugs.
研究了一种使用花的水提取物作为“天然工厂”来制备生物功能化银纳米颗粒（AgNPs）的单步方案。生物还原过程后，银离子与花的水提取物之间的反应导致形成红棕色胶体溶液。XRD图谱显示了AgNPs的面心立方晶体结构，FE-SEM表征显示其呈现球形形态。FTIR研究确定了参与有效包覆AgNPs的不同官能团。这证实了植物还原的AgNPs具有良好的稳定性，并且通过DLS测量了颗粒的大小。合成的AgNPs对MCF7显示出有效的细胞毒性潜力，抑制浓度（IC）记录为7.19μg/mL。AO/EB染色也证实了AgNPs的凋亡作用。该研究提供了初步证据，表明生物合成的AgNPs可用于开发新型抗癌药物。
