Chahardoli Azam, Qalekhani Farshad, Hajmomeni Pouria, Shokoohinia Yalda, Fattahi Ali
Department of Biology, Faculty of Science, Razi University, Kermanshah, 6714414971, Iran.
Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Sci Rep. 2025 Jan 7;15(1):1186. doi: 10.1038/s41598-024-82349-z.
In the current research, we developed a safe method using Iranian yarrow extract for the synthesis of silver nanoparticles (IY-AgNPs) as reducing and stabilizing agents in different conditions. The prepared and stabilized IY-AgNPs under optimal conditions were characterized using FT-IR, XRD, TEM, and UV-vis techniques. Also, the blood-clotting, hemolytic, antioxidant, bactericidal and, fungicidal properties, cytotoxicity effects and inhibition of protein denaturation efficiency of IY-AgNPs were assessed in vitro. The stabilized IY-AgNPs with spherical shape and an average particle size of 19. 25 ± 7.9 nm did not show any hemolytic potential below 1000 µg/mL. These hemo-compatible NPs showed good blood-clotting ability by reducing clotting time (6 min relative to the control). These particles excellently inhibited the denaturation of bovine serum albumin (BSA) by 69.3-80.7% at concentrations ranging from 31.25 to 500 µg/mL compared to a reference drug. The outcomes showed that the IC50 values of IY-AgNPs were below 12.5 µg/mL against A375 cells and between 25 and 50 µg/mL against MCF-7 cancer cells. In addition, IY-AgNPs were bactericidal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus (especially), and were fungicidal against Candida albicans. Biosynthesized IY-AgNPs indicated a significant antioxidant activity (63.2%) at a concentration of 350 µg/mL. These attained results suggested that bio/hemo-compatible IY-AgNPs may be a promising candidate for applications in the medicinal fields (particularly for wound healing) as anti-bleeding, antimicrobial, antioxidant, anti-inflammatory, and anticancer agents.
在当前的研究中，我们开发了一种安全的方法，使用伊朗蓍草提取物在不同条件下作为还原剂和稳定剂来合成银纳米颗粒（IY-AgNPs）。在最佳条件下制备并稳定的IY-AgNPs使用傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、透射电子显微镜（TEM）和紫外可见光谱（UV-vis）技术进行表征。此外，还在体外评估了IY-AgNPs的凝血、溶血、抗氧化、杀菌和杀真菌特性、细胞毒性作用以及抑制蛋白质变性的效率。形状为球形且平均粒径为19.25±7.9nm的稳定IY-AgNPs在浓度低于1000μg/mL时未显示出任何溶血潜力。这些血液相容性纳米颗粒通过缩短凝血时间（相对于对照组为6分钟）显示出良好的凝血能力。与参考药物相比，这些颗粒在浓度范围为31.25至500μg/mL时能出色地抑制牛血清白蛋白（BSA）变性69.3%-80.7%。结果表明，IY-AgNPs对A375细胞的半数抑制浓度（IC50）值低于12.5μg/mL，对MCF-7癌细胞的IC50值在25至50μg/mL之间。此外，IY-AgNPs对大肠杆菌、铜绿假单胞菌尤其是金黄色葡萄球菌具有杀菌作用，对白色念珠菌具有杀真菌作用。生物合成的IY-AgNPs在浓度为350μg/mL时表现出显著的抗氧化活性（63.2%）。这些结果表明，生物/血液相容性的IY-AgNPs作为抗出血、抗菌、抗氧化、抗炎和抗癌剂，在医学领域（特别是伤口愈合）的应用中可能是一个有前景的候选者。
