Hashemi Sheikh Shabani Somayeh, Seyed Hasan Tehrani Sahar, Rabiei Zohreh, Tahmasebi Enferadi Sattar, Vannozzi Gian Paolo
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Dipartimenti di Sienze Agraria, Universita degli Studi di Udine, Udine, Italy.
Biotechnol Rep (Amst). 2015 Oct 27;8:138-143. doi: 10.1016/j.btre.2015.08.007. eCollection 2015 Dec.
This research was done to evaluate the induction of apoptosis in MDA-MB-231 breast cancer cell line by 's extract, in which a significant amount of ß-carbolines is included. The apoptosis incidence was assessed through Annexin-V-Flous kit. The expressions of genes through which intrinsic apoptosis pathway are involved, Bax, Bcl-2, Bid, and Puma, over the genes the expressions of which are linked to extrinsic apoptosis pathway, TRAIL, Caspase8, p21, and p53, were examined by RT-PCR and Real-time PCR. The results demonstrate that the extract decreases the growth rate of the cancer cell line through inducing apoptosis mechanism. As long as the expression of anti-apoptosis Bcl-2 gen reduced dramatically, an over-expression in Bax and Puma genes was monitored indicating activation of intrinsic apoptosis pathway. A notable over-expression observed with TRAIL and Caspase8 genes as well as Bid gene. The latter is an intermediate for both intrinsic and extrinsic pathways of apoptosis.
本研究旨在评估含有大量β-咔啉的[提取物名称]提取物对MDA-MB-231乳腺癌细胞系凋亡的诱导作用。通过Annexin-V-Flous试剂盒评估凋亡发生率。通过RT-PCR和实时PCR检测参与内源性凋亡途径的基因Bax、Bcl-2、Bid和Puma的表达，以及与外源性凋亡途径相关的基因TRAIL、Caspase8、p21和p53的表达。结果表明，该提取物通过诱导凋亡机制降低癌细胞系的生长速率。由于抗凋亡Bcl-2基因的表达显著降低，监测到Bax和Puma基因的过表达，表明内源性凋亡途径被激活。TRAIL、Caspase8基因以及Bid基因也观察到显著的过表达。后者是凋亡内源性和外源性途径的中间体。
