Boonhok Rachasak, Sangkanu Suthinee, Chuprom Julalak, Srisuphanunt Mayuna, Norouzi Roghayeh, Siyadatpanah Abolghasem, Mirzaei Farzaneh, Mitsuwan Watcharapong, Wisessombat Sueptrakool, de Lourdes Pereira Maria, Rahmatullah Mohammed, Wilairatana Polrat, Wiart Christophe, Ling Lim Chooi, Dolma Karma G, Nissapatorn Veeranoot
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.
Research Excellence Center for Innovation and Health Products (RECIHP), School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team) and World Union for Herbal Drug Discovery (WUHeDD), Walailak University, Nakhon Si Thammarat 80160, Thailand.
Pathogens. 2021 Jul 4;10(7):842. doi: 10.3390/pathogens10070842.
, a well-known medicinal plant, has been used for several therapeutic purposes as it contains numerous pharmacological active compounds. Our study reported an anti-parasitic activity of seed extract against . The stress induced by the extract on the surviving trophozoites for encystation and vacuolization was examined by microscopy, and transcriptional expression of autophagy-related genes was investigated by quantitative PCR. Our results showed that the surviving trophozoites were not transformed into cysts, and the number of trophozoites with enlarged vacuoles were not significantly different from that of untreated control. Molecular analysis data demonstrated that the mRNA expression of tested ATG genes, i.e., ATG3, ATG8b, and ATG16, was at a basal level along the treatment. However, upregulation of ATG16 at 24 h post treatment was observed, which may indicate an autophagic activity of this protein in response to the stress. Altogether, these data revealed the anti- activity of extract and indicated the association of autophagy mRNA expression and cyst formation under the extract stress, representing a promising plant for future drug development. However, further identification of an active compound and a study of autophagy at the protein level are needed.
一种著名的药用植物，因其含有众多药理活性化合物而被用于多种治疗目的。我们的研究报道了该植物种子提取物对[寄生虫名称]的抗寄生虫活性。通过显微镜检查提取物对存活滋养体诱导包囊化和空泡化的应激情况，并通过定量PCR研究自噬相关基因的转录表达。我们的结果表明，存活的滋养体未转化为包囊，且空泡增大的滋养体数量与未处理对照相比无显著差异。分子分析数据表明，所测试的自噬相关基因（即ATG3、ATG8b和ATG16）的mRNA表达在整个处理过程中处于基础水平。然而，在处理后24小时观察到ATG16上调，这可能表明该蛋白在应激反应中有自噬活性。总之，这些数据揭示了该植物提取物的抗[寄生虫名称]活性，并表明在提取物应激下自噬mRNA表达与包囊形成之间的关联，使其成为未来药物开发的有前景植物。然而，需要进一步鉴定活性化合物并在蛋白质水平研究自噬。
