长链非编码RNA TUG1在骨肉瘤中表达上调并促进细胞增殖。
LncRNA TUG1 is upregulated and promotes cell proliferation in osteosarcoma.
作者信息
Yun-Bo Feng, Xiao-Po Liu, Xiao-Li Li, Guo-Long Cao, Pei Zhang, Fa-Ming Tian
机构信息
Should be: Tangshan Gongren Hospital, Tangshan Hebei Province 063000, PR China.
Department of orthopaedics, Tangshan City workers Hospital, Tangshan Hebei Province 063000 PR China.
出版信息
Open Med (Wars). 2016 May 30;11(1):163-167. doi: 10.1515/med-2016-0031. eCollection 2016.
OBJECTIVE
To examine the expression and function of long non-coding RNA () in human osteosarcoma cells.
METHODS
Real-time quantitive PCR was used to detect the transcription level of in a series of osteosarcoma cell lines. Knockdown of in U2OS cells was carried out by transient transfection of siRNAs. MTT assay was performed to access the cell growth rates. Afterwards, RNA and protein of these cells were extracted to analyze the transfection efficient as well as the expression of other molecules.
RESULTS
Compared to the normal cell line, exhibited a significant upregulation in osteosarcoma cells. Phenotyping analysis showed the growth-promotion activity of , since knockdown of resulted in declined proliferation. We also found that AKT phosphorylation was impaired after was inhibited, suggesting that the AKT pathway was involved in the regulation of in U2OS cells.
CONCLUSION
Our data provided evidence that was upregulated and acted as a possible oncogene via positively regulating cell proliferation in osteosarcoma cells.
目的
检测长链非编码RNA（ ）在人骨肉瘤细胞中的表达及功能。
方法
采用实时定量PCR检测一系列骨肉瘤细胞系中 的转录水平。通过瞬时转染小干扰RNA（siRNAs）敲低U2OS细胞中的 。进行MTT试验以评估细胞生长速率。之后，提取这些细胞的RNA和蛋白质以分析转染效率以及其他分子的表达。
结果
与正常细胞系相比， 在骨肉瘤细胞中显著上调。表型分析显示 具有促生长活性，因为敲低 导致增殖下降。我们还发现抑制 后AKT磷酸化受损，提示AKT信号通路参与U2OS细胞中 的调控。
结论
我们的数据表明 在骨肉瘤细胞中上调，并通过正向调节细胞增殖可能作为一种癌基因发挥作用。
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