Zeng Leping, He Yaojia, Jiao Liangcheng, Li Kai, Yan Yunjun
Key Laboratory of Molecular Biophysics of the Ministry of Education, China-EU Institute for Clean and Renewable Energy, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China.
Appl Biochem Biotechnol. 2017 Nov;183(3):778-791. doi: 10.1007/s12010-017-2463-y. Epub 2017 Mar 29.
To reduce industrial production cost, cheap and easily available rapeseed oil deodorizer distillates were used as feedstock to prepare biodiesel in this study. As a result, liquid forms of Candida rugosa lipase and Rhizopus oryzae lipase (ROL) were functioned as new and effective catalysts with biodiesel yield of 92.63% for 30 h and 94.36% for 9 h, respectively. Furthermore, the synergetic effect between the two lipases was employed to enhance biodiesel yield with a result of 98.16% in 6 h under optimized conditions via response surface methodology. The obtained conversion rate surpassed both yields of the individual two lipases and markedly shortened the reaction time. The resultant optimal conditions were ROL ratio 0.84, water content 46 wt% (w/w), reaction temperature 34 °C, and reaction time 6 h.
为降低工业生产成本，本研究采用廉价易得的菜籽油脱臭馏出物作为原料制备生物柴油。结果表明，皱褶假丝酵母脂肪酶和米根霉脂肪酶（ROL）的液体形式作为新型高效催化剂，生物柴油产率分别在30小时时为92.63%，9小时时为94.36%。此外，通过响应面法，利用两种脂肪酶之间的协同作用提高生物柴油产率，在优化条件下6小时产率达到98.16%。所得转化率超过了两种脂肪酶单独作用时的产率，且显著缩短了反应时间。最佳条件为ROL比例0.84、含水量46 wt%（w/w）、反应温度34℃和反应时间6小时。
