Dolph P J, Racaniello V, Villamarin A, Palladino F, Schneider R J
Department of Biochemistry, New York University Medical Center, New York 10016.
J Virol. 1988 Jun;62(6):2059-66. doi: 10.1128/JVI.62.6.2059-2066.1988.
The adenovirus tripartite leader is a 200-nucleotide 5' noncoding region that is found on all late viral mRNAs. This segment is required for preferential translation of viral mRNAs at late times during infection. Most tripartite leader-containing mRNAs appear to exhibit little if any requirement for intact cap-binding protein complex, a property previously established only for uncapped poliovirus mRNAs and capped mRNAs with minimal secondary structure. The tripartite leader also permits the translation of mRNAs in poliovirus-infected cells in the apparent absence of active cap-binding protein complex and does not require any adenovirus gene products for this activity. The preferential translation of viral late mRNAs may involve this unusual property.
腺病毒三联体前导序列是一个200个核苷酸的5'非编码区，存在于所有晚期病毒mRNA上。该片段是感染后期病毒mRNA优先翻译所必需的。大多数含有三联体前导序列的mRNA似乎对完整的帽结合蛋白复合物几乎没有（如果有的话）需求，这一特性以前仅在无帽的脊髓灰质炎病毒mRNA和具有最小二级结构的有帽mRNA中得到证实。三联体前导序列还能在明显缺乏活性帽结合蛋白复合物的情况下，使脊髓灰质炎病毒感染细胞中的mRNA进行翻译，并且该活性不需要任何腺病毒基因产物。病毒晚期mRNA的优先翻译可能涉及这一不同寻常的特性。
