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腺病毒三联前导序列可改变受感染细胞内非腺病毒信使核糖核酸的核代谢和胞质代谢。

The adenovirus tripartite leader sequence can alter nuclear and cytoplasmic metabolism of a non-adenovirus mRNA within infected cells.

作者信息

Moore M A, Shenk T

机构信息

Department of Molecular Biology, Princeton University, NJ 08544.

出版信息

Nucleic Acids Res. 1988 Mar 25;16(5):2247-62. doi: 10.1093/nar/16.5.2247.

DOI:10.1093/nar/16.5.2247
PMID:3357776
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338213/
Abstract

All mRNAs encoded by the adenovirus major late transcription unit share a common 5' noncoding region, 200 nucleotides in length, termed the tripartite leader sequence. To assess function of the tripartite leader, recombinant viruses were prepared which carried either a bona fide herpes simplex virus thymidine kinase gene or a modified thymidine kinase gene whose normal 5' noncoding domain was replaced with the adenovirus leader sequence. The tripartite leader simultaneously decreased the nuclear half-life and increased the cytoplasmic half-life of the thymidine kinase-specific mRNA. The tripartite leader stabilized the non-adenovirus mRNA only within the environment of an adenovirus-infected cell during the late phase of the infectious cycle.

摘要

腺病毒主要晚期转录单位编码的所有mRNA都共享一个共同的5'非编码区，长度为200个核苷酸，称为三联前导序列。为了评估三联前导序列的功能，制备了重组病毒，这些病毒携带一个真正的单纯疱疹病毒胸苷激酶基因或一个修饰的胸苷激酶基因，其正常的5'非编码结构域被腺病毒前导序列所取代。三联前导序列同时降低了胸苷激酶特异性mRNA的核半衰期并增加了其细胞质半衰期。三联前导序列仅在感染周期的后期，在腺病毒感染细胞的环境中稳定非腺病毒mRNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6af1/338213/e4dc715e19f2/nar00148-0375-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6af1/338213/81a6186ad3c6/nar00148-0374-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6af1/338213/e4dc715e19f2/nar00148-0375-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6af1/338213/81a6186ad3c6/nar00148-0374-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6af1/338213/e4dc715e19f2/nar00148-0375-a.jpg

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本文引用的文献

1
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Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6. doi: 10.1073/pnas.78.4.2072.
2
The primary transcription unit of the mouse beta-major globin gene.小鼠β-珠蛋白基因的初级转录单位。
Cell. 1981 Feb;23(2):585-93. doi: 10.1016/0092-8674(81)90154-9.
3
The stability of early adenovirus mRNA is controlled by the viral 72 kd DNA-binding protein.早期腺病毒信使核糖核酸的稳定性由病毒72千道尔顿DNA结合蛋白控制。
哺乳动物细胞中的信使核糖核酸稳定性
Microbiol Rev. 1995 Sep;59(3):423-50. doi: 10.1128/mr.59.3.423-450.1995.
4
Adenovirus vector expressing functional herpes simplex virus ICP0.表达功能性单纯疱疹病毒ICP0的腺病毒载体
J Virol. 1988 Dec;62(12):4544-53. doi: 10.1128/JVI.62.12.4544-4553.1988.
5
Adenovirus early region 4 stimulates mRNA accumulation via 5' introns.腺病毒早期区域4通过5'内含子刺激mRNA积累。
Proc Natl Acad Sci U S A. 1990 Dec;87(24):9543-7. doi: 10.1073/pnas.87.24.9543.
Cell. 1981 Nov;26(3 Pt 1):371-9. doi: 10.1016/0092-8674(81)90206-3.
4
Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.使用特异性克隆的cDNA探针检测α-和β-微管蛋白以及细胞质β-和γ-肌动蛋白基因的数量和进化保守性。
Cell. 1980 May;20(1):95-105. doi: 10.1016/0092-8674(80)90238-x.
5
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Cell. 1983 Jul;33(3):695-703. doi: 10.1016/0092-8674(83)90012-0.
6
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7
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8
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10
Construction in vitro and rescue of a thymidine kinase-deficient deletion mutation of herpes simplex virus.单纯疱疹病毒胸苷激酶缺陷型缺失突变体的体外构建与拯救
Nature. 1980 May 29;285(5763):333-5. doi: 10.1038/285333a0.