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腺病毒三联前导序列增强感染后期mRNA的翻译。

Adenovirus tripartite leader sequence enhances translation of mRNAs late after infection.

作者信息

Logan J, Shenk T

出版信息

Proc Natl Acad Sci U S A. 1984 Jun;81(12):3655-9. doi: 10.1073/pnas.81.12.3655.

DOI:10.1073/pnas.81.12.3655
PMID:6587381
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC345277/
Abstract

A series of adenovirus type 5 variants was constructed to probe the function of the tripartite leader sequence, a 200-nucleotide, 5' noncoding sequence carried on the majority of late viral mRNAs. Recombinant plasmids were constructed that carried the major late transcriptional control region followed by portions of the tripartite leader sequence fused to the E1A coding region. These modified E1A genes were then rebuilt into intact viral chromosomes, replacing the corresponding wild-type region. The leader segments had no effect on the translation of E1A mRNAs early after infection, but the tripartite leader significantly enhanced (5-fold) the efficiency with which the mRNAs were translated late after infection.

摘要

构建了一系列5型腺病毒变体，以探究三联前导序列的功能。该序列是一段200个核苷酸的5'非编码序列，存在于大多数晚期病毒mRNA上。构建了重组质粒，其携带主要晚期转录控制区，随后是与E1A编码区融合的三联前导序列的部分片段。然后将这些修饰的E1A基因重建到完整的病毒染色体中，取代相应的野生型区域。前导片段在感染后早期对E1A mRNA的翻译没有影响，但三联前导序列在感染后晚期显著提高了（5倍）mRNA的翻译效率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/05201e9168b1/pnas00613-0058-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/49ed053aae73/pnas00613-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/7b35da026c19/pnas00613-0057-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/392bbdfb00ca/pnas00613-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/b656151920c8/pnas00613-0058-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/a0b431682ef1/pnas00613-0058-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/05201e9168b1/pnas00613-0058-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/49ed053aae73/pnas00613-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/7b35da026c19/pnas00613-0057-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/392bbdfb00ca/pnas00613-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/b656151920c8/pnas00613-0058-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/a0b431682ef1/pnas00613-0058-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69d8/345277/05201e9168b1/pnas00613-0058-d.jpg

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本文引用的文献

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Nature. 1980 Jun 19;285(5766):575-7. doi: 10.1038/285575a0.
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Enhanced expression of adenovirus transforming proteins.腺病毒转化蛋白的表达增强。
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Nucleotide sequences from the adenovirus-2 genome.来自腺病毒2型基因组的核苷酸序列。
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Global Transcriptome Analyses of Cellular and Viral mRNAs during HAdV-C5 Infection Highlight New Aspects of Viral mRNA Biogenesis and Cytoplasmic Viral mRNA Accumulations.HAdV-C5 感染过程中细胞和病毒 mRNA 的全球转录组分析突显了病毒 mRNA 生成和细胞质病毒 mRNA 积累的新方面。
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PLoS One. 2019 Apr 3;14(4):e0214882. doi: 10.1371/journal.pone.0214882. eCollection 2019.
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Effect of adenovirus infection on transgene expression under the adenoviral MLP/TPL and the CMVie promoter/enhancer in CHO cells.腺病毒感染对中国仓鼠卵巢细胞中腺病毒主要晚期启动子/三联前导序列及巨细胞病毒立即早期启动子/增强子调控下转基因表达的影响。
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Adenoviromics: Mining the Human Adenovirus Species D Genome.腺病毒组学：挖掘人类腺病毒D种基因组
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The adenovirus L4-22K protein has distinct functions in the posttranscriptional regulation of gene expression and encapsidation of the viral genome.腺病毒 L4-22K 蛋白在基因表达的转录后调控和病毒基因组的包装中具有不同的功能。
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Functional analysis of the nucleotide sequence surrounding the cap site for adenovirus type 5 region E1A messenger RNAs.腺病毒5型E1A信使核糖核酸帽位点周围核苷酸序列的功能分析。
J Mol Biol. 1983 Jul 15;167(4):809-22. doi: 10.1016/s0022-2836(83)80112-0.
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