Booth Judith K, Page Jonathan E, Bohlmann Jörg
Michael Smith Laboratories, University of British Columbia, East Mall, Vancouver, B.C., Canada, V6T 1Z4.
Anandia Laboratories, Lower Mall, Vancouver, B.C., Canada, V6T 1Z4.
PLoS One. 2017 Mar 29;12(3):e0173911. doi: 10.1371/journal.pone.0173911. eCollection 2017.
Cannabis (Cannabis sativa) plants produce and accumulate a terpene-rich resin in glandular trichomes, which are abundant on the surface of the female inflorescence. Bouquets of different monoterpenes and sesquiterpenes are important components of cannabis resin as they define some of the unique organoleptic properties and may also influence medicinal qualities of different cannabis strains and varieties. Transcriptome analysis of trichomes of the cannabis hemp variety 'Finola' revealed sequences of all stages of terpene biosynthesis. Nine cannabis terpene synthases (CsTPS) were identified in subfamilies TPS-a and TPS-b. Functional characterization identified mono- and sesqui-TPS, whose products collectively comprise most of the terpenes of 'Finola' resin, including major compounds such as β-myrcene, (E)-β-ocimene, (-)-limonene, (+)-α-pinene, β-caryophyllene, and α-humulene. Transcripts associated with terpene biosynthesis are highly expressed in trichomes compared to non-resin producing tissues. Knowledge of the CsTPS gene family may offer opportunities for selection and improvement of terpene profiles of interest in different cannabis strains and varieties.
大麻（Cannabis sativa）植株在腺毛中产生并积累富含萜烯的树脂，腺毛在雌花序表面大量存在。不同单萜和倍半萜的组合是大麻树脂的重要成分，因为它们决定了一些独特的感官特性，还可能影响不同大麻品系和品种的药用品质。对大麻品种“菲诺拉”（Finola）腺毛的转录组分析揭示了萜烯生物合成各个阶段的序列。在TPS-a和TPS-b亚家族中鉴定出9种大麻萜烯合酶（CsTPS）。功能表征鉴定出了单萜和倍半萜TPS，其产物共同构成了“菲诺拉”树脂的大部分萜烯，包括主要化合物如β-月桂烯、（E）-β-罗勒烯、（-）-柠檬烯、（+）-α-蒎烯、β-石竹烯和α-葎草烯。与萜烯生物合成相关的转录本在腺毛中的表达高于非树脂产生组织。CsTPS基因家族的知识可能为选择和改善不同大麻品系和品种中感兴趣的萜烯谱提供机会。
