Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products, Faculty of Pharmacy, Monastir, Avanue Avicenne, 5000 Monastir, Tunisia.
Department of Clinical Sciences and Community Health, University of Milan and Epidemiology Unit, Fondazione IRCCS Ca' GrandaOspedale Maggiore Policlinico, Via S. Barnaba, 8-20122 Milan, Italy.
Ann Work Expo Health. 2017 Apr 1;61(3):333-343. doi: 10.1093/annweh/wxw030.
In this study, urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), as biomarker of oxidative DNA damage, was evaluated in Tunisian electric steel foundry workers and was associated with polycyclic aromatic hydrocarbon (PAH) exposure. Ninety-three healthy male workers were enrolled in the study; 8-oxodG was assessed by liquid chromatography-triple quadrupole mass spectrometry. Exposure to PAHs was evaluated by measuring 16 urinary PAHs (U-PAHs) and 8 monohydroxylated metabolites (OHPAHs). The median 8-oxodG level for all subjects was 3.20 µg l-1 (1.85 µg g-1 creatinine). No correlation between 8-oxodG and 1-hydroxypyrene or any other OHPAH was found. Significant linear correlations between 8-oxodG and some U-PAHs were found, particularly urinary acenaphthylene (r = 0.249), phenanthrene (r = 0.327), anthracene (r = 0.357), fluoranthene (r = 0.248), and pyrene (r = 0.244). Multiple regression analyses confirmed that urinary phenanthrene, anthracene, and naphthalene (the latter with a non-linear relationship) were predictors of 8-oxodG; job title, but not smoking, was a determinant of 8-oxodG; the variance explained by these models was up to 20%. The oxidative DNA damage assessed by urinary 8-oxodG was moderate and in the range of values reported in other occupational fields or in the general population. The results of this study indicate that the investigated biomarkers of PAH exposure were only minor contributors to urinary 8-oxodG.
在这项研究中，我们评估了尿 8-氧代-7,8-二氢-2'-脱氧鸟苷（8-oxodG）作为氧化 DNA 损伤的生物标志物，它与多环芳烃（PAH）暴露有关。我们招募了 93 名健康的男性工人参与这项研究；通过液相色谱-三重四极杆质谱法评估 8-oxodG。通过测量 16 种尿多环芳烃（U-PAHs）和 8 种单羟基代谢物（OHPAHs）来评估 PAH 暴露情况。所有受试者的 8-oxodG 中位数水平为 3.20µg l-1（1.85µg g-1 肌酐）。未发现 8-oxodG 与 1-羟基芘或任何其他 OHPAH 之间存在相关性。发现 8-oxodG 与某些 U-PAHs 之间存在显著的线性相关性，特别是尿苊烯（r=0.249）、菲（r=0.327）、蒽（r=0.357）、荧蒽（r=0.248）和芘（r=0.244）。多元回归分析证实，尿中的菲、蒽和萘（后者呈非线性关系）是 8-oxodG 的预测因子；工作岗位，而不是吸烟，是 8-oxodG 的决定因素；这些模型解释的方差高达 20%。通过尿 8-oxodG 评估的氧化 DNA 损伤程度适中，处于其他职业领域或一般人群报告的数值范围内。本研究结果表明，所研究的 PAH 暴露生物标志物对尿 8-oxodG 的贡献很小。
