Feldman D, Goldstein A L, Cox D C, Grimley P M
Department of Pharmacology and Chemotherapy, Hoffmann-La Roche Inc., Nutley, New Jersey.
Lab Invest. 1988 May;58(5):584-9.
Cultured human umbilical vein endothelial cells and human thoracic aorta endothelial cells were treated with 500, 1,000, and 2,000 IU/ml of recombinant leukocyte alpha-interferon for 2, 4, and 7 days. Tubuloreticular inclusions developed in treated human umbilical vein endothelial cells and human thoracic aorta endothelial cells. The size and number of tubuloreticular inclusions observed correlated with the dose and duration of treatment. Interferon treatment inhibited the rate of proliferation of both human umbilical vein endothelial cells and human thoracic aorta endothelial cells in a dose- and duration-dependent manner. Induction of the interferon-associated enzyme, 2'-5' oligoadenylate synthetase, also occurred. Since the endothelium is exposed to endogenous interferon present in certain pathologic conditions and to exogenous interferon administered in the treatment of several neoplastic or viral diseases, there is an increased need to understand the various effects of interferon on the endothelial cell.
将培养的人脐静脉内皮细胞和人胸主动脉内皮细胞分别用500、1000和2000 IU/ml的重组白细胞α-干扰素处理2天、4天和7天。在处理过的人脐静脉内皮细胞和人胸主动脉内皮细胞中出现了管网状包涵体。观察到的管网状包涵体的大小和数量与治疗剂量和持续时间相关。干扰素处理以剂量和时间依赖性方式抑制人脐静脉内皮细胞和人胸主动脉内皮细胞的增殖速率。还诱导了干扰素相关酶2'-5'寡腺苷酸合成酶。由于内皮细胞在某些病理条件下会接触内源性干扰素，并且在治疗几种肿瘤或病毒性疾病时会接触外源性干扰素，因此越来越需要了解干扰素对内皮细胞的各种影响。
