Yan Hui, Ji Xunda, Li Jing, Zhang Lei, Zhao Peiquan
Department of Ophthalmology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China.
Department of Ophthalmology, Tongji University Affiliated Yangpu Hospital, Shanghai 200090, P.R. China.
Oncol Lett. 2017 Feb;13(2):827-833. doi: 10.3892/ol.2016.5507. Epub 2016 Dec 16.
The present study aimed to investigate the expression of cluster of differentiation 82 (KAI1), a gene involved in the suppression of tumor metastasis, in human retinoblastoma (RB) tissue and to study the effect of KAI1 expression on RB cell migration and invasion. KAI1 expression was examined in 26 patients with non-invasive and invasive retinoblastoma using reverse transcription-quantitative polymerase chain reaction and western blot analysis. A lentiviral vector containing KAI1 cDNA was used to transfect the two RB cell lines, HXO-Rb44-Gl and Y79. Following successful transfection, the migratory and invasive capacity of the two RB cell lines was evaluated using a Transwell migration assay. KAI1 expression was observed to be downregulated in invasive RB compared to non-invasive RB. The migratory and invasive capacities of KAI1 transfected cell lines were significantly decreased compared to those of the control cells. KAI1 may be involved in retinoblastoma metastasis, and increased expression of KAI1 significantly inhibits the metastatic ability of RB cells .
本研究旨在调查参与抑制肿瘤转移的基因——分化簇82（KAI1）在人视网膜母细胞瘤（RB）组织中的表达情况，并研究KAI1表达对RB细胞迁移和侵袭的影响。采用逆转录-定量聚合酶链反应和蛋白质免疫印迹分析，检测了26例非侵袭性和侵袭性视网膜母细胞瘤患者的KAI1表达。使用含有KAI1 cDNA的慢病毒载体转染两种RB细胞系HXO-Rb44-Gl和Y79。成功转染后，使用Transwell迁移试验评估两种RB细胞系的迁移和侵袭能力。观察到与非侵袭性RB相比，侵袭性RB中KAI1表达下调。与对照细胞相比，KAI1转染细胞系的迁移和侵袭能力显著降低。KAI1可能参与视网膜母细胞瘤转移，KAI1表达增加可显著抑制RB细胞的转移能力。
