Department of Pathology, First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Bengbu City, 233000, Anhui Province, PR China.
Arch Iran Med. 2012 Nov;15(11):707-12.
This study investigates the expression of CD82/KAI1 and epithelial-cadherin (E-cad) in non-small cell lung cancer (NSCLC).
Tissues of resected primary NSCLC and normal lung tissue were investigated. Protein expression was detected with immunohistochemical staining. mRNA expression levels of CD82/KAI1 and E-cad were determined by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) after mRNA extraction.
The expression of CD82/KAI1 and E-cad was significantly lower in NSCLC compared to normal lung tissue (P < 0.01). CD82/KAI1 and E-cad mRNA and protein expression were found to be in close relationship with the grade of differentiation, lymph node metastasis and pathologic tumor-node-metastasis (pTNM) stages, and survival in NSCLC (P < 0.01), but no relationship was observed with gender, diameter, type, histological type and age (P > 0.05). Expression of mRNA CD82/KAI1 and E-cad were consistent with their proteins (P < 0.01), and there was a significant relationship between expression of CD82/KAI1 and E-cad. The survival rate of the CD82/KAI1-positive and CD82/KAI1-negative groups was significantly different (P < 0.01). In addition, the survival rate was significantly different between the E-cad-positive and E-cad-negative groups. pTNM stages and positive expression of CD82/KAI1 and E-cad were independent prognostic factors of NSCLC (P < 0.05).
Lower expression of CD82/KAI1 and E-cad was found in NSCLC compared to normal lung tissue. Decreased expression of CD82/KAI1 and E-cad was closely related to cellular differentiation, pTNM stages, invasion and metastasis.
本研究旨在探讨 CD82/KAI1 和上皮钙黏附素（E-cad）在非小细胞肺癌（NSCLC）中的表达。
采用免疫组织化学染色法检测切除的原发性 NSCLC 组织和正常肺组织中的蛋白表达情况，通过半定量逆转录聚合酶链反应（RT-PCR）检测 CD82/KAI1 和 E-cad 的 mRNA 表达水平。
与正常肺组织相比，NSCLC 中 CD82/KAI1 和 E-cad 的表达显著降低（P<0.01）。CD82/KAI1 和 E-cad 的 mRNA 和蛋白表达与分化程度、淋巴结转移、病理肿瘤-淋巴结-转移（pTNM）分期以及 NSCLC 的生存情况密切相关（P<0.01），但与性别、直径、类型、组织学类型和年龄无关（P>0.05）。CD82/KAI1 和 E-cad 的 mRNA 表达与其蛋白表达一致（P<0.01），且二者之间存在显著相关性。CD82/KAI1 阳性和 CD82/KAI1 阴性组的生存率有显著差异（P<0.01）。此外，E-cad 阳性和 E-cad 阴性组的生存率也存在显著差异。pTNM 分期以及 CD82/KAI1 和 E-cad 的阳性表达是非小细胞肺癌的独立预后因素（P<0.05）。
与正常肺组织相比，NSCLC 中 CD82/KAI1 和 E-cad 的表达水平较低。CD82/KAI1 和 E-cad 的表达下调与细胞分化、pTNM 分期、侵袭和转移密切相关。
