微小RNA-149靶向特异性蛋白1以抑制人舌鳞状细胞癌细胞的增殖和运动。
MicroRNA-149 targets specificity protein 1 to suppress human tongue squamous cell carcinoma cell proliferation and motility.
作者信息
Chen Jingxin, Chen Jimin, Li Weizhong
机构信息
Department of Oral and Maxillofacial Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510000, P.R. China; Department of Oral and Maxillofacial Surgery, Hainan Province People's Hospital, Haikou, Hainan 570011, P.R. China.
Department of Pathology, Hainan Province People's Hospital, Haikou, Hainan 570011, P.R. China.
出版信息
Oncol Lett. 2017 Feb;13(2):851-856. doi: 10.3892/ol.2016.5527. Epub 2016 Dec 20.
The expression and function of microRNA-149 have been studied in numerous types of cancer. However, thus far, there are no studies of microRNA-149 in tongue squamous cell carcinoma (TSCC). The present study investigated the expression, biological function and molecular mechanism of microRNA-149 in TSCC , discussing whether it may be a therapeutic biomarker of TSCC in the future. In the present study, microRNA-149 expression in TSCC tissues, matched normal adjacent tissues, TSCC cell lines and normal gingival epithelial cells were analyzed using quantitative polymerase chain reaction. Following transfection with microRNA-149 mimics, cell proliferation, migration and invasion assays, a luciferase assay and western blotting were performed. The present study found that the expression of microRNA-149 was significantly decreased in TSCC tissues and cell lines compared with matched normal tissue and normal gingival epithelial cells, respectively. In addition, it was also demonstrated that microRNA-149 inhibited cell proliferation, migration and invasion by directly targeting specificity protein 1. Therefore, the results suggested that microRNA-149 may be a novel target for TSCC therapy in the future.
微小RNA-149的表达及功能已在多种癌症类型中得到研究。然而，迄今为止，尚无关于微小RNA-149在舌鳞状细胞癌（TSCC）中的研究。本研究调查了微小RNA-149在TSCC中的表达、生物学功能及分子机制，探讨其未来是否可能成为TSCC的治疗生物标志物。在本研究中，采用定量聚合酶链反应分析TSCC组织、配对的正常相邻组织、TSCC细胞系及正常牙龈上皮细胞中微小RNA-149的表达。转染微小RNA-149模拟物后，进行细胞增殖、迁移和侵袭实验、荧光素酶实验及蛋白质印迹分析。本研究发现，与配对的正常组织及正常牙龈上皮细胞相比，微小RNA-149在TSCC组织和细胞系中的表达分别显著降低。此外，还证明微小RNA-149通过直接靶向特异性蛋白1抑制细胞增殖、迁移和侵袭。因此，结果表明微小RNA-149未来可能成为TSCC治疗的新靶点。
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