Wang Jian, Yan Guang-Peng, Guo Chao, Li Jun
Dept. of Oral and Maxillofacial Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China.
Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Shihezi University, Shihezi 832000, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2020 Feb 1;38(1):11-16. doi: 10.7518/hxkq.2020.01.003.
The expression of microRNA-125b in tongue squamous cell carcinoma (TSCC) was detected and analyzed for its relationship with the clinicopathological features of TSCC.
Real time fluorescence-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of microRNA-125b in 35 TSCC tissues and adjacent normal tissues from 35 TSCC cases. The relationship between the expression of microRNA-125b in TSCC tissues and the clinicopathological features of patients with TSCC was analyzed. In situ hybridization (ISH) was used to detect the expression level of microRNA-125b gene in the TSCC tissues and adjacent normal tissues.
RT-qPCR results showed that the relative expression levels of microRNA-125b in the TSCC issues was 2.32±0.69, and that of normal tissues was 0.87±0.32. The statistical results showed that the expression level of microRNA-125b was significantly higher in the TSCC tissues than in the normal tissues (P<0.001). The expression level of microRNA-125b in the TSCC tissues was not significantly correlated with age, gender, pathological grade, and lymph node metastasis but was positively correlated with TNM stage. Patients with high TNM stage had high microRNA-125b expression levels (P<0.05). The ISH results showed that the expression levels of microRNA-125b in the TSCC tissues were 0.010±0.003, and that of normal tissues was 0.004±0.001. The expression levels of microRNA-125b in the 35 TSCC tissues were significantly higher than those in the normal tissues (P<0.05).
MicroRNA-125b is highly expressed in TSCC and associated with TNM stage, suggesting that high microRNA-125b expression may be involved in the development of TSCC.
检测微小RNA-125b（microRNA-125b）在舌鳞状细胞癌（TSCC）中的表达，并分析其与TSCC临床病理特征的关系。
采用实时荧光定量聚合酶链反应（RT-qPCR）检测35例TSCC患者的35例TSCC组织及癌旁正常组织中microRNA-125b的表达。分析TSCC组织中microRNA-125b的表达与TSCC患者临床病理特征的关系。采用原位杂交（ISH）检测TSCC组织及癌旁正常组织中microRNA-125b基因的表达水平。
RT-qPCR结果显示，TSCC组织中microRNA-125b的相对表达水平为2.32±0.69，正常组织为0.87±0.32。统计学结果显示，TSCC组织中microRNA-125b的表达水平显著高于正常组织（P<0.001）。TSCC组织中microRNA-125b的表达水平与年龄、性别、病理分级和淋巴结转移无显著相关性，但与TNM分期呈正相关。TNM分期高的患者microRNA-125b表达水平高（P<0.05）。ISH结果显示，TSCC组织中microRNA-125b的表达水平为0.010±0.003，正常组织为0.004±0.001。35例TSCC组织中microRNA-125b的表达水平显著高于正常组织（P<0.05）。
MicroRNA-125b在TSCC中高表达并与TNM分期相关，提示microRNA-125b高表达可能参与TSCC的发生发展。
