BcefI, a new type IIS restriction endonuclease.
作者信息
Venetianer P, Orosz A
机构信息
Institute of Biochemistry, Biological Research Center, Szeged, Hungary.
出版信息
Nucleic Acids Res. 1988 Apr 11;16(7):3053-60. doi: 10.1093/nar/16.7.3053.
Abstract
A new Type IIS restriction endonuclease was identified, partially purified and characterized from a Bacillus cereus subsp. fluorescens strain. The enzyme recognizes the nonpalindromic sequence ACGGC and cleaves at a distance from it. The cleavage appears to occur with a +/- 1 basepair uncertainty. Thus the cleavage and recognition site is as shown below: ACGGC(N)11-13 TGCCG(N)12-14.
摘要
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