do Nascimento Mônica Helena Monteiro, Ferreira Mariselma, Malmonge Sônia Maria, Lombello Christiane Bertachini
Human and Natural Sciences Center, ABC Federal University, Santo André, São Paulo, Brazil.
Engineering, Modelling and Applied Social Sciences Center, ABC Federal University, Santo André, São Paulo, Brazil.
J Mater Sci Mater Med. 2017 May;28(5):68. doi: 10.1007/s10856-017-5875-x. Epub 2017 Mar 29.
Tissue engineering involves the development of new materials or devices capable of specific interactions with biological tissues, searching the use of biocompatible materials as scaffolds for in vitro cell growth, and functional tissue development, that is subsequently implanted into patient. The aim of the current study was to evaluate the initial aspects of cell interaction with the polymeric biomaterials blends based on hyaluronic acid with chitosan. The hypothesis approach involves synthesis and analysis of swelling and thermal degradation (thermal gravimetric analysis) of the polymer blend; and Vero cell interaction with the biomaterial, through analysis of cytotoxicity, adhesion and cell morphology. The blend resulted in a biomaterial with a high swelling ratio that can allow nutrient distribution and absorption. The thermal gravimetric analysis results showed that the blend had two stages of degradation at temperatures very close to those observed for pure polymers, confirming that the physical mixing of hydrogels occurred, resulting in the presence of both hyaluronic acid and chitosan in the blend. The evaluation of indirect cytotoxicity showed that the blend was non cytotoxic for Vero cells, and the quantitative analysis performed with the MTT could verify a cell viability of 98%. The cells cultured on the blend showed adhesion, spreading and proliferation on this biomaterial, distinguished from the pattern of the control cells. These results showed that the blends produced from hyaluronic acid and chitosan hydrogels are promising for applications in tissue engineering, aiming at future cartilaginous tissue.
组织工程涉及开发能够与生物组织发生特定相互作用的新材料或装置，探索使用生物相容性材料作为体外细胞生长的支架以及功能性组织的发育，随后将其植入患者体内。本研究的目的是评估基于透明质酸与壳聚糖的聚合物生物材料共混物与细胞相互作用的初始方面。假设方法包括聚合物共混物的溶胀和热降解（热重分析）的合成与分析；以及通过细胞毒性、粘附和细胞形态分析，研究Vero细胞与生物材料的相互作用。该共混物形成了一种具有高溶胀率的生物材料，能够实现营养物质的分布和吸收。热重分析结果表明，该共混物在与纯聚合物非常接近的温度下有两个降解阶段，证实了水凝胶发生了物理混合，导致共混物中同时存在透明质酸和壳聚糖。间接细胞毒性评估表明，该共混物对Vero细胞无细胞毒性，用MTT进行的定量分析可验证细胞活力为98%。在该共混物上培养的细胞在这种生物材料上表现出粘附、铺展和增殖，与对照细胞的模式不同。这些结果表明，由透明质酸和壳聚糖水凝胶制成的共混物在组织工程应用中具有前景，目标是未来的软骨组织。
