Benson Kathrin, Galla Hans-Joachim, Kehr Nermin Seda
Institut für Biochemie, Westfälische Wilhelms-Universität Münster, Wilhelm-Klemm-Str. 2, D-48149, Münster, Germany.
Macromol Biosci. 2014 Jun;14(6):793-8. doi: 10.1002/mabi.201300485. Epub 2014 Feb 10.
Alginate hydrogels functionalized with D-, or L-penicillamine (D-, L-PEN-Alg) are used as new 3D scaffolds for cell adhesion studies. The cells recognize and show different adhesion properties in the respective 3D hydrogel scaffolds. C-6-glioma and endothelial cells show higher affinity to the D-PEN than to the L-PEN functionalized 3D alginate hydrogel scaffold. The cultivated cells are harvested from the hydrogel and are reused, for example, for cell growth experiments on 2D surfaces to prove their viability as well.
用D-或L-青霉胺功能化的海藻酸盐水凝胶（D-、L-PEN-Alg）用作细胞黏附研究的新型3D支架。细胞在各自的3D水凝胶支架中识别并表现出不同的黏附特性。C-6胶质瘤细胞和内皮细胞对D-PEN功能化的3D海藻酸盐水凝胶支架的亲和力高于对L-PEN功能化的支架。从水凝胶中收获培养的细胞并重复使用，例如用于二维表面的细胞生长实验，以证明它们的活力。
