Department of Bioengineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea.
Macromol Biosci. 2012 Nov;12(11):1502-13. doi: 10.1002/mabi.201200148. Epub 2012 Sep 10.
Synthetic substrates with defined chemical and structural characteristics may potentially be prepared to mimic the living ECM to regulate cell adhesion and growth. Hydrogels with cell-adhesive peptides (0.28 ± 0.03 nmol peptide cm(-2) , TTA-R-0.5; and 0.91 ± 0.12 nmol peptide cm(-2) , TTA-R-2.0) and/or micro-scaled topographical patterns (10, 25, and 80 µm grooves) are prepared using enzymatic polymerization. The adherent morphology and proliferation of C2C12 skeletal myoblasts and human aortic smooth muscle cells (hAoSM) on the hydrogels are studied. The newly developed hydrogels may be useful in investigating the roles of cell adhesion and substrate surface properties in the communication of adherent cells with the ECM.
具有明确化学和结构特征的合成底物可能被制备出来以模拟活细胞外基质来调节细胞黏附和生长。具有细胞黏附肽（0.28 ± 0.03 nmol 肽/cm²，TTA-R-0.5；和 0.91 ± 0.12 nmol 肽/cm²，TTA-R-2.0）和/或微尺度形貌（10、25 和 80 µm 沟槽）的水凝胶是使用酶聚合制备的。研究了 C2C12 骨骼肌母细胞和人主动脉平滑肌细胞（hAoSM）在水凝胶上的黏附形态和增殖。新开发的水凝胶可用于研究细胞黏附和基底表面性质在黏附细胞与细胞外基质的通讯中的作用。
