Takeda K, Kawamura M
Department of Biology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
J UOEH. 1988 Mar 1;10(1):59-62. doi: 10.7888/juoeh.10.59.
Phenylmethanesulfonyl fluoride (PMSF) enabled vanadate to catalyze the oxidation of NADH. Superoxide dismutase and manganese inhibited the oxidation, suggesting that superoxide anion played a crucial role. Since PMSF is widely used to protect enzymes from proteolytic cleavage when they are purified, attention should be paid when the toxicity of vanadate on the enzyme, whose activities are monitored by the oxidation of NADH, is evaluated or when vanadate dependent NADH oxidase is purified and assayed.
苯甲基磺酰氟（PMSF）能使钒酸盐催化NADH的氧化。超氧化物歧化酶和锰抑制了这种氧化，这表明超氧阴离子起到了关键作用。由于PMSF在纯化酶时被广泛用于保护其免受蛋白水解切割，因此在评估钒酸盐对通过NADH氧化来监测活性的酶的毒性时，或者在纯化和检测依赖钒酸盐的NADH氧化酶时，应予以注意。
