钒酸盐增强NADH氧化作用机制的进一步研究。

Further studies of the mechanism of the enhancement of NADH oxidation by vanadate.

作者信息

Liochev S, Fridovich I

出版信息

J Free Radic Biol Med. 1985;1(4):287-92. doi: 10.1016/0748-5514(85)90133-3.

Abstract

O2-., whether generated photochemically, or introduced as a solution of KO2 in a nonprotic solvent, caused rapid oxidation of NADH in the presence, but not in the absence of vanadate. Superoxide dismutase inhibited this vanadate-stimulated oxidation of NADH, while catalase had no effect. This NADH oxidation appears to be a free-radical chain reaction whose average chain length was estimated to be 15 in the photochemical system. Vanadate stimulation of NADH oxidation by biological membranes can now be viewed as a sensitive indicator of O2-. production by those membranes.

摘要

超氧阴离子（O2-.），无论是通过光化学方式生成，还是作为超氧化钾在非质子溶剂中的溶液引入，在有钒酸盐存在时会导致NADH快速氧化，但在无钒酸盐时则不会。超氧化物歧化酶抑制了这种由钒酸盐刺激的NADH氧化，而过氧化氢酶则没有作用。这种NADH氧化似乎是一个自由基链反应，在光化学系统中其平均链长估计为15。现在可以将钒酸盐对生物膜NADH氧化的刺激视为这些膜产生超氧阴离子（O2-.）的一个敏感指标。