Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan.
Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
Sci Rep. 2017 Mar 30;7:45607. doi: 10.1038/srep45607.
Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the transplanted FND-labeled pcMSCs in organs and tissues of the miniature pigs after intravenous administration. The method is applicable to single-cell imaging and quantitative tracking of human stem/progenitor cells in rodents and other animal models as well.
细胞治疗是治疗人类疾病的一种很有前途的策略。虽然细胞首次被用于治疗目的可以追溯到 19 世纪，但一直缺乏通用且可靠的方法来研究各种动物模型中移植细胞的生物分布和相关药代动力学，以便进行临床前评估。在这里，我们提出了一个新的平台，使用白蛋白结合的荧光纳米金刚石（FND）作为生物相容性和光稳定性标记物，通过磁调制对迷你猪中的人胎盘绒毛膜蜕膜衍生间充质干细胞（pcMSC）进行定量跟踪。利用这种无背景检测技术和时间门控荧光成像，我们能够精确地确定静脉给药后 FND 标记的 pcMSC 在迷你猪的器官和组织中的数量和位置。该方法适用于在啮齿动物和其他动物模型中单细胞成像和人干细胞/祖细胞的定量跟踪。
