嗜神经病毒在恶性胶质瘤中的患病率及其肿瘤调节潜力。
Prevalence of Neurotropic Viruses in Malignant Glioma and Their Onco-Modulatory Potential.
作者信息
Strojnik Tadej, Duh Darja, Lah Tamara T
机构信息
Department of Neurosurgery, University Clinical Centre Maribor, Maribor, Slovenia
Faculty of Medicine, University of Maribor, Maribor, Slovenia.
出版信息
In Vivo. 2017 Mar-Apr;31(2):221-229. doi: 10.21873/invivo.11049.
BACKGROUND
the association between infectious agents and tumour aetiology is relevant in about 20% of cases.
PATIENTS AND METHODS
We tested high-grade glioma tissues from 45 patients for the presence of viral nucleic acids of six herpes viruses, human adenoviruses (A-G), and two neurotropic human viruses (enteroviruses, tick-borne encephalitis virus). Real-time polymerase chain reaction was used with immunolabelling.
RESULTS
Three species of herpes viruses were detected: HSV-2, Epstein-Barr virus (EBV), HHV-6, and one human enterovirus. Plasma of these patients was not infected with viruses. In sera of patients, low HSV-1 and HSV-2 immunoreactivity were found in five cases, although these were not detected in their tumour tissue.
CONCLUSION
Certain common viruses (HSV-1, HSV-2, EBV, human cytomegalovirus) are chronically present in the sera of patients with glioblastoma, but not necessarily in their tissues. Possibly both are associated with glioma progression, as we only found viruses in glioblastoma multiforme, but not in lower stages of glioma. Low titres of viruses in the blood indicate chronic viral virulence.
背景
在约20%的病例中，感染因子与肿瘤病因之间存在关联。
患者与方法
我们检测了45例患者的高级别胶质瘤组织，以查找六种疱疹病毒、人腺病毒（A - G）以及两种嗜神经性人类病毒（肠道病毒、蜱传脑炎病毒）的病毒核酸。采用实时聚合酶链反应并结合免疫标记法。
结果
检测到三种疱疹病毒：单纯疱疹病毒2型（HSV - 2）、爱泼斯坦 - 巴尔病毒（EBV）、人疱疹病毒6型（HHV - 6），以及一种人类肠道病毒。这些患者的血浆未感染病毒。在患者血清中，五例检测到低水平的HSV - 1和HSV - 2免疫反应性，尽管在其肿瘤组织中未检测到。
结论
某些常见病毒（HSV - 1、HSV - 2、EBV、人巨细胞病毒）长期存在于胶质母细胞瘤患者的血清中，但不一定存在于其组织中。两者可能都与胶质瘤进展有关，因为我们仅在多形性胶质母细胞瘤中发现病毒，而在较低级别的胶质瘤中未发现。血液中病毒滴度低表明病毒具有慢性毒力。
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