琼枝麒麟菜硫酸寡糖通过上调调节性 T（Treg）细胞来降低动物模型中的食物过敏反应。
Eucheuma cottonii Sulfated Oligosaccharides Decrease Food Allergic Responses in Animal Models by Up-regulating Regulatory T (Treg) Cells.
机构信息
College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University , 43 Yindou Road, Xiamen 361021, Fujian, People's Republic of China.
Southern Regional Research Center, U.S. Department of Agriculture, Agriculture Research Service , 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, United States.
出版信息
J Agric Food Chem. 2017 Apr 19;65(15):3212-3222. doi: 10.1021/acs.jafc.7b00389. Epub 2017 Apr 6.
In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used to test the anti-food allergy activity of ESO. The effects of ESO on the regulatory T (Treg) cells and bone marrow-derived mast cells (BMMCs) were investigated by flow cytometry. The results of in vivo assay showed that ESO decreased the levels of mast cell protease-1 and histamine and inhibited the levels of specific IgE by 77.7%. In addition, the production of interleukin (IL)-4 and IL-13 was diminished in the ESO groups compared to the non-ESO-treated group. Furthermore, ESO could up-regulate Treg cells by 22.2-97.1%. In conclusion, ESO decreased the allergy response in mice by reducing basophil degranulation, up-regulating Treg cells via Forkhead box protein 3 (Foxp3), and releasing IL-10. ESO may have preventive and therapeutic potential in allergic disease.
在本研究中，研究了角叉菜硫酸寡糖（ESO）的抗食物过敏活性。ESO 通过酶解和柱层析纯化获得。使用 RBL-2H3 细胞和 BALB/c 小鼠模型来测试 ESO 的抗食物过敏活性。通过流式细胞术研究了 ESO 对调节性 T（Treg）细胞和骨髓来源的肥大细胞（BMMCs）的影响。体内实验结果表明，ESO 使肥大细胞蛋白酶-1 和组氨酸的水平降低了 77.7%，并抑制了特异性 IgE 的水平。此外，与非 ESO 处理组相比，ESO 组的白细胞介素（IL）-4 和 IL-13 的产生减少。此外，ESO 可通过上调 Treg 细胞 22.2-97.1%。总之，ESO 通过减少嗜碱性粒细胞脱颗粒、通过叉头框蛋白 3（Foxp3）上调 Treg 细胞以及释放白细胞介素 10，减少了小鼠的过敏反应。ESO 在过敏疾病中可能具有预防和治疗潜力。
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