Bhat Mohammad Ashraf, Parry Manzoor Ahmad, Nissar Saniya, Sameer Aga Syed, Bhat Imtiyaz A, Shah Zafar A, Rasool Roohi
Department of Nephrology, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India.
Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, 190011, India.
BMC Nephrol. 2017 Mar 30;18(1):111. doi: 10.1186/s12882-017-0526-5.
Cytokines have been found to be the important mediators during renal graft outcome. Therefore, we designed this study to investigate the role of recipients' IL-1 β promoter (-511) and IL-1 β exon-5 (+3954) polymorphisms with the risk of graft outcome.
We enrolled one hundred recipients of living-related renal transplants together with the age and sex matched controls from the healthy population not having any renal abnormality for this study. Genotype frequencies of the IL-1 β promoter (-511) and IL-1 β exon-5 (+3954) were analyzed using PCR-RFLP technique.
Our results revealed significant differences in the healthy control group and patient group in IL 1β +3954 (p < 0.001). The frequency of variant type TT genotype was higher in RE group as compared to SGF and showed 4 fold risk of rejection (OR = 4.54, p < 0.069) although p value was not significant. The frequency of wild type CC genotype and CT was not significant (p value 0.89 and 0.74 respectively).
Our findings suggest that there is a prevalence of mutated allele of IL-1 gene cluster in our population, which may be responsible for renal dysfunction.
细胞因子已被发现是肾移植结果的重要介质。因此，我们设计了本研究，以探讨受体白细胞介素-1β启动子（-511）和白细胞介素-1β外显子5（+3954）多态性与移植结果风险之间的关系。
我们招募了100名亲属活体肾移植受体以及年龄和性别匹配的健康对照者，这些对照者没有任何肾脏异常。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术分析白细胞介素-1β启动子（-511）和白细胞介素-1β外显子5（+3954）的基因型频率。
我们的结果显示，白细胞介素-1β+3954在健康对照组和患者组之间存在显著差异（p<0.001）。与移植成功组相比，移植排斥组中变异型TT基因型的频率更高，尽管p值不显著，但显示出4倍的排斥风险（OR=4.54，p<0.069）。野生型CC基因型和CT基因型的频率无显著差异（p值分别为0.89和0.74）。
我们的研究结果表明，我们的人群中白细胞介素-1基因簇的突变等位基因普遍存在，这可能是肾功能障碍的原因。
