Yao Zhi-Cheng, Jin Li-Jie, Ahmad Zeeshan, Huang Jie, Chang Ming-Wei, Li Jing-Song
Key Laboratory for Biomedical Engineering of Education Ministry of China, Hangzhou 310027, People's Republic of China; Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, 310027, People's Republic of China.
Leicester School of Pharmacy, De Montfort, University, The Gateway, Leicester, LE1 9BH, UK.
Int J Pharm. 2017 May 30;524(1-2):148-158. doi: 10.1016/j.ijpharm.2017.03.064. Epub 2017 Mar 28.
Ganoderma lucidum polysaccharide (GLP) is a functional food source deployed in preventative medicine. However, applications utilizing GLP are limited due to oxidative and acidic environmental damage. Advances in preserving GLP structure (and therefore function), in situ, will diversify their applications within biomedical fields (drug and antibacterial active delivery via the enteral route). In this study, GLP loaded sodium alginate (NaAlg) micro-particles (size range 225-355μm) were generated using the electrospray (ES) process. The loading capacity and encapsulation efficiency of GLP for composite particles (collected at different temperatures) were ∼23% and 71%, respectively. The collection substrate (CaCl, 1-20w/v%) concentration was explored and preliminary findings indicated a 10w/v% solution to be optimal. The process was further modified by manipulating the collection environment temperature (∼25 to 50°C). Based on this, NaAlg/GLP micro-particles were engineered with variable surface morphologies (porous and crinkled), without effecting the chemical composition of either material (GLP and NaAlg). In-vitro release studies demonstrated pH responsive release rates. Modest release of GLP from micro-particles in simulated gastric fluid (pH ∼1.7) was observed, while rapid release was exhibited under simulated intestinal conditions (pH ∼7.4). Release of GLP from NaAlg beads was the greatest from samples prepared at elevated environmental temperatures. These findings demonstrate a facile route to fabricate GLP-NaAlg loaded micro-particles with various shapes, surface topographies and release characteristics via a one-step ES process.
灵芝多糖（GLP）是一种应用于预防医学的功能性食品原料。然而，由于氧化和酸性环境的破坏，GLP的应用受到限制。原位保存GLP结构（进而保持其功能）方面的进展将使其在生物医学领域（通过肠内途径进行药物和抗菌活性递送）的应用更加多样化。在本研究中，采用电喷雾（ES）工艺制备了负载GLP的海藻酸钠（NaAlg）微粒（尺寸范围为225 - 355μm）。复合微粒（在不同温度下收集）对GLP的负载量和包封率分别约为23%和71%。研究了收集底物（CaCl，1 - 20w/v%）的浓度，初步结果表明10w/v%的溶液为最佳。通过控制收集环境温度（约25至50°C）对该工艺进行了进一步改进。在此基础上，制备了具有可变表面形态（多孔和起皱）的NaAlg/GLP微粒，且不影响任何一种材料（GLP和NaAlg）的化学成分。体外释放研究表明其具有pH响应释放速率。在模拟胃液（pH约1.7）中观察到GLP从微粒中有适度释放，而在模拟肠道条件（pH约7.4）下则表现出快速释放。从NaAlg珠粒中释放的GLP在较高环境温度下制备的样品中释放量最大。这些研究结果表明，通过一步ES工艺可以轻松制备具有各种形状、表面形貌和释放特性的负载GLP - NaAlg的微粒。
