Division of Tissue Engineering & Regeneration Technologies, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, 695012, India.
Division of Toxicology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, 695012, India.
Int J Biol Macromol. 2017 Nov;104(Pt B):1936-1945. doi: 10.1016/j.ijbiomac.2017.03.142. Epub 2017 Mar 27.
The current study investigates the potential of chitosan-hyaluronic acid dialdehyde hydrogels for in vivo cartilage regeneration following two different approaches: Gel alone for cartilage regeneration or combination of chondrocytes and gel for cartilage repair. Critical size osteochondral defects were created in knee joints of Newzealand White rabbits. Allogenic rabbit chondrocytes were encapsulated in hydrogels and gel or gel+cells were implanted in defects aseptically. The regenerated cartilage was analyzed after 12 weeks of implantation. The morphological scoring indicates that repair tissue was formed in all the animals by 12 weeks irrespective of whether they were sham, received gel, or gel with cells as implant. However the repair tissue formed in sham appeared fibrous and opaque, where as those that received gel had texture similar to the surrounding native cartilage and animals with gel+cells showed varied response. Histology staining, score distribution and immunostaining for collagen Type II showed animals that received gel alone as the implant had a mixture of hyaline and fibro cartilage. The animals with cell encapsulated gels had more fibrous cells with weak staining for collagen type II. There was no significant enhancement in the quality of regenerated cartilage in presence of encapsulated chondrocytes.
本研究探讨了壳聚糖-透明质酸二醛水凝胶在两种不同方法下用于体内软骨再生的潜力：单独凝胶用于软骨再生或软骨细胞与凝胶结合用于软骨修复。在新西兰白兔的膝关节中创建临界大小的骨软骨缺损。同种异体兔软骨细胞被包封在水凝胶中，凝胶或凝胶+细胞被无菌植入缺陷部位。植入 12 周后分析再生软骨。形态学评分表明，无论动物是假手术组、接受凝胶还是凝胶+细胞作为植入物，所有动物在 12 周内均形成了修复组织。然而，假手术组形成的修复组织呈纤维状且不透明，而接受凝胶的组织具有与周围天然软骨相似的质地，且接受凝胶+细胞的动物表现出不同的反应。组织学染色、胶原 II 评分分布和免疫染色表明，单独接受凝胶作为植入物的动物具有混合的透明软骨和纤维软骨。包封软骨细胞的凝胶中的动物具有更多的纤维细胞，胶原 II 染色较弱。在包封软骨细胞的存在下，再生软骨的质量没有显著提高。
