Hao Xuefeng, Chen Changming, Chen Guoju, Cao Bihao, Lei Jianjun
Department of Biology, Taiyuan Normal University, Taiyuan 030012, Shanxi Province, People's Republic of China.
J Genet. 2017 Mar;96(1):25-31. doi: 10.1007/s12041-016-0735-x.
Our previous study on the genic male sterile-fertile line 114AB of Capsicum annuum indicated a diversity of differentially expressed cDNA fragments in fertile and sterile lines. In this study, a transcript-derived fragment (TDF), male fertile 4 (CaMF4) was chosen for further investigation to observe that this specific fragment accumulates in the flower buds of the fertile line. The full genomic DNA sequence of CaMF4 was 894 bp in length, containing two exons and one intron, and the complete coding sequence encoded a putative 11.53 kDa protein of 109 amino acids. The derived protein of CaMF4 shared similarity with the members of PGPS/D3 protein family. The expression of CaMF4 was detected in both the flower buds at stage 8 and open flowers of the male fertile line. In contrast to this observation, expression of CaMF4 was not detected in any organs of the male sterile line. Further analysis revealed that CaMF4 was expressed particularly in anthers of the fertile line. Our results suggest that CaMF4 is an anther-specific gene and might be indispensable for anther or pollen development in C. annuum.
我们之前对辣椒雄性不育-可育系114AB的研究表明，可育系和不育系中存在多种差异表达的cDNA片段。在本研究中，选择了一个转录本衍生片段（TDF），雄性可育4（CaMF4）进行进一步研究，以观察该特定片段在可育系花芽中积累。CaMF4的完整基因组DNA序列长度为894 bp，包含两个外显子和一个内含子，完整的编码序列编码一个推定的11.53 kDa、含109个氨基酸的蛋白质。CaMF4的推导蛋白与PGPS/D3蛋白家族成员具有相似性。在雄性可育系的8期花芽和开放花中均检测到CaMF4的表达。与此观察结果相反，在雄性不育系的任何器官中均未检测到CaMF4的表达。进一步分析表明，CaMF4在可育系的花药中特异性表达。我们的结果表明，CaMF4是一个花药特异性基因，可能对辣椒花药或花粉发育不可或缺。
