Kokut Süleyman, Atay İnci Meltem, Uz Efkan, Akpinar Abdullah, Demirdaş Arif
Clinic of Psychiatry, Serik Public Hospital, Antalya, Turkey.
Department of Psychiatry, Süleyman Demirel University, Faculty of Medicine, Isparta, Turkey.
Noro Psikiyatr Ars. 2015 Jun;52(2):124-127. doi: 10.5152/npa.2015.7321. Epub 2015 Jun 1.
It was reported that the genetic susceptibility of major depressive disorder (MDD) is related with genetic polymorphisms. The aim of this study was to investigate the possible association of the genotype and allele frequencies of Ser49Gly and Arg389Gly polymorphisms in MDD by comparing them with healthy subjects.
A total of 144 patients with MDD diagnosed according to Diagnostic and Statistical Manual of Mental Disorders, 4th Edition (DSM-IV) criteria and 105 healthy controls were included in the study. Polymerase chain reaction (PCR) with restriction fragment length polymorphism (RFLP) was used for genotyping.
Of the 144 participants in the MDD group, 77 (53.5%) had homozygous wild type (AA), 57 (39.6%) had heterozygous type (AG), and 10 (6.9%) had mutant (GG) genotype for Ser49Gly, whereas 75 (52.1%) had homozygous wild type (GG), 59 (41.0%) had heterozygous (GC) type, and 10 (6.9%) had mutant homozygous (CC) genotype for Gly386Arg. There were no significant difference in the allele and genotype frequencies of the beta-1-adrenergic receptor (ADRB1) gene for Ser49Gly and Arg389Gly polymorphisms after comparing with healthy controls (p=0.626; p=0.863 and p=0.625; p=0.914).
The results of our study did not reveal a major effect of the polymorphism of Ser49Gly and Gly389Arg in the ADRB1 gene in MDD. Further studies with larger sample size are required to elucidate the role of other beta-1 adrenergic gene polymorphisms in MDD.
据报道，重度抑郁症（MDD）的遗传易感性与基因多态性有关。本研究旨在通过将MDD患者与健康受试者进行比较，探讨β-1肾上腺素能受体（ADRB1）基因Ser49Gly和Arg389Gly多态性的基因型和等位基因频率的可能关联。
本研究纳入了144例根据《精神疾病诊断与统计手册》第4版（DSM-IV）标准诊断的MDD患者和105例健康对照。采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）进行基因分型。
在MDD组的144名参与者中，77名（53.5%）具有Ser49Gly的纯合野生型（AA），57名（39.6%）具有杂合型（AG），10名（6.9%）具有突变型（GG）基因型；而对于Gly386Arg，75名（52.1%）具有纯合野生型（GG），59名（41.0%）具有杂合型（GC），10名（6.9%）具有突变纯合型（CC）基因型。与健康对照相比，ADRB1基因Ser49Gly和Arg389Gly多态性的等位基因和基因型频率无显著差异（p = 0.626；p = 0.863和p = 0.625；p = 0.914）。
我们的研究结果未揭示ADRB1基因Ser49Gly和Gly389Arg多态性在MDD中的主要作用。需要进一步进行更大样本量的研究，以阐明其他β-1肾上腺素能基因多态性在MDD中的作用。
