Toma Luciano, Di Luca Marco, Mancini Fabiola, Severini Francesco, Mariano Carmela, Nicolai Giancarlo, Laghezza Masci Valentina, Ciervo Alessandra, Fausto Anna Maria, Cacciò Simone Mario
Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, Rome, Italy.
Dipartimento di Prevenzione, Azienda Sanitaria Locale di Viterbo, Viterbo, Italy.
Ann Ist Super Sanita. 2017 Jan-Mar;53(1):30-34. doi: 10.4415/ANN_17_01_07.
In 2012-2013, an investigation was carried out in the Viterbo province, Lazio region, on ticks and tick-borne Apicomplexan protozoa of the Babesia and Theileria genera. This followed the reporting of high density of ticks by soldiers operating in a military shooting range, and the signaling by owners and local veterinary authorities of several cases of babesiosis among cattle.
A total of 422 ticks were collected from 35 heads, whereas 96 ticks were collected by dragging. Ticks were identified as Rhipicephalus (Boophilus) annulatus Say (n = 373), Rhipicephalus bursa Canestrini & Fanzago (n = 63), Rhipicephalus sanguineus/turanicus (n = 32), Hyalomma marginatum Koch (n = 49) and Dermacentor marginatus Sulzer, 1776 (n = 1). A randomly selected sample of ticks (235 from animals and 36 by dragging) was analyzed using molecular methods to detect species of Babesia and Theileria.
In total, 11 ticks collected from animals (4.7%) and two ticks (5.5%) collected by dragging were positive. Sequencing of PCR products of the small subunit ribosomal RNA gene revealed Babesia caballi (n = 2), Babesia bigemina (n = 3), Theileria sergenti/buffeli/orientalis (n = 7) and Theileria equi (n = 1). None of the detected species has been associated with human infection.
2012年至2013年，在拉齐奥大区维泰博省对蜱以及巴贝斯属和泰勒虫属的蜱传顶复门原虫进行了调查。此次调查是在军事靶场执行任务的士兵报告蜱虫高密度出现，以及牲畜所有者和当地兽医当局报告多起牛巴贝斯虫病病例之后展开的。
从35头牛身上共采集了422只蜱，另外通过拖拽采集了96只蜱。蜱被鉴定为环形扇头蜱（Rhipicephalus (Boophilus) annulatus Say，n = 373）、囊形扇头蜱（Rhipicephalus bursa Canestrini & Fanzago，n = 63）、血红扇头蜱/图兰扇头蜱（Rhipicephalus sanguineus/turanicus，n = 32）、边缘璃眼蜱（Hyalomma marginatum Koch，n = 49）和边缘革蜱（Dermacentor marginatus Sulzer，1776，n = 1）。使用分子方法对随机选取的蜱样本（235只采自动物，36只通过拖拽采集）进行分析，以检测巴贝斯虫属和泰勒虫属的种类。
总共采自动物的11只蜱（4.7%）和通过拖拽采集的2只蜱（5.5%）呈阳性。小亚基核糖体RNA基因的PCR产物测序显示有马巴贝斯虫（n = 2）、双芽巴贝斯虫（n = 3）、瑟氏泰勒虫/水牛泰勒虫/东方泰勒虫（n = 7）和马泰勒虫（n = 1）。所检测到的种类均未与人类感染相关联。
