Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China.
Department of Prosthodontics, Peking University School and Hospital of Stomatology; National Engineering Laboratory for Digital and Material Technology of Stomatology; Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
Sci Rep. 2017 Mar 31;7:45655. doi: 10.1038/srep45655.
The purpose of this study is to investigate electrospinning poly(L-lactic acid) (PLLA) nanofibrous scaffold with different contents of amorphous calcium phosphate (ACP), which is suitable for using in bone regeneration through sustained release of calcium and orthophosphate ions. Three groups of nanofibrous scaffolds, ACP-free PLLA, ACP-5 wt%/PLLA and ACP-10 wt%/PLLA, are developed and characterized by scanning electron microscopy and gel permeation chromatography. Calcium and phosphate colorimetric assay kits are used to test ions released from scaffold during hydrolytic degradation. The results show ACP-5 wt%/PLLA and ACP-10 wt%/PLLA scaffolds have relatively high degradation rates than ACP-free PLLA group. The bioactivity evaluation further reveals that ACP-5 wt%/PLLA scaffold presents more biocompatible feature with pre-osteoblast cells and significant osteogenesis ability of calvarial bone defect. Due to the facile preparation method, sustained calcium and orthophosphate release behavior, and excellent osteogenesis capacity, the presented ACP/PLLA nanofibrous scaffold has potential applications in bone tissue engineering.
本研究旨在通过持续释放钙和正磷酸盐离子，研究不同含量无定形磷酸钙（ACP）的聚 L-乳酸（PLLA）纳米纤维支架，以适用于骨再生。通过扫描电子显微镜和凝胶渗透色谱法对无 ACP 的 PLLA、ACP-5wt%/PLLA 和 ACP-10wt%/PLLA 三种纳米纤维支架进行了表征。使用钙和磷酸盐比色试剂盒测试支架在水解降解过程中释放的离子。结果表明，ACP-5wt%/PLLA 和 ACP-10wt%/PLLA 支架的降解速率明显高于无 ACP 的 PLLA 组。生物活性评价进一步表明，ACP-5wt%/PLLA 支架与前成骨细胞具有更好的生物相容性和显著的颅骨骨缺损成骨能力。由于其制备方法简单、持续释放钙和正磷酸盐的行为以及优异的成骨能力，所提出的 ACP/PLLA 纳米纤维支架在骨组织工程中有潜在的应用前景。
