Massart C, Hody B, Condé D, Leclech G, Edan G, Nicol M
Laboratoire de Biochimie A, Service d'O.R.L., C.H.U. de Pontchaillou, Rennes, France.
Mol Cell Endocrinol. 1988 Apr;56(3):227-34. doi: 10.1016/0303-7207(88)90065-2.
Cultures of human thyroid follicles embedded in collagen gel were performed to investigate certain functional properties under bovine thyrotropin (TSH) stimulation. Follicles obtained from normal glands responded to increasing concentrations of TSH administered on day 4 in culture and for 3 days by increased amounts of cyclic AMP (cAMP), thyroglobulin (Tg) and triiodothyronine (T3) and by decreased levels of thyroxine (T4). Effect was maximal at 2000 microU/ml TSH (cAMP) or 200 microU/ml (Tg, T3, T4). When methimazole or propylthiouracil (PTU) were added, the T3 levels decreased. Follicle lumens contained a periodic acid-Schiff substance which was identified by immunoreaction as Tg. Thyroid follicles obtained from Graves' disease glands gave modified results with an earlier and intensified T3 response and no increase in Tg. These data show that (1) Tg and T3 are secretory products of functional follicles giving a cAMP-mediated response to TSH. (2) The detected T3 also derives from T4 5'-deiodination inhibited by PTU. (3) Intensified T3 response in Graves' follicles is probably due to enhanced conversion of T4 to T3.
为了研究在牛促甲状腺激素（TSH）刺激下的某些功能特性，对包埋于胶原凝胶中的人甲状腺滤泡进行了培养。从正常腺体获取的滤泡对培养第4天及之后3天给予的浓度不断增加的TSH作出反应，表现为环磷酸腺苷（cAMP）、甲状腺球蛋白（Tg）和三碘甲状腺原氨酸（T3）的量增加，以及甲状腺素（T4）水平降低。在2000微单位/毫升TSH（cAMP）或200微单位/毫升（Tg、T3、T4）时效应最大。当加入甲巯咪唑或丙硫氧嘧啶（PTU）时，T3水平降低。滤泡腔内含有一种经免疫反应鉴定为Tg的过碘酸希夫物质。从格雷夫斯病腺体获取的甲状腺滤泡给出了不同的结果，T3反应更早且更强，而Tg没有增加。这些数据表明：（1）Tg和T3是功能性滤泡的分泌产物，对TSH产生cAMP介导的反应。（2）检测到的T3也来源于被PTU抑制的T4 5'-脱碘作用。（3）格雷夫斯滤泡中增强的T3反应可能是由于T4向T3的转化增强。
