Yamashita H, Noguchi S, Murakami N, Adachi M, Yasuoka Y, Wakiya S, Kitamura H
Department of Pathology, Oita Medical University, Japan.
Pathol Int. 1994 Dec;44(12):827-31. doi: 10.1111/j.1440-1827.1994.tb01679.x.
Thyrocytes obtained from patients with Graves' disease were cultured for 3 days. This was followed by culture with 10 mU/mL thyroid stimulating hormone (TSH) (TSH group), TSH and sodium iodide (Nal group), or without (control group) for 3 additional days. On the 8th culture day, the amounts of intra- and extra-cellular cyclic adenosine monophosphate (cAMP), extracellular cAMP and thyroglobulin (TG), peroxidase (PO) activity, and cell numbers were measured. The amounts of intra- and extra-cellular cAMP correlated well. TSH increased the values of cAMP, TG and PO to levels higher than those of the control group. As the amount of Nal added to the medium increased, these values decreased. Addition of 10(-5) mol/L Nal lowered the value of cAMP only. When 10(-4) mol/L Nal was added, these three levels were lower than those of the TSH group and the value of cAMP was almost equal to that of the control group. On cell number, no difference was found between the cells cultured with TSH, TSH and Nal, and without TSH or Nal. When the thyrocytes were cultured with 1 mmol/L dibutyryl cAMP sodium salt or 8-bromoadenosine 3',5'-cyclic monophosphate instead of TSH, 10(-4) mol/L Nal did not lower the values of thyroglobulin and peroxidase activity. These results suggest that the Nal blocks the intracellular signal transduction provoked by TSH, only at the cAMP production level.
从格雷夫斯病患者身上获取的甲状腺细胞培养3天。随后分别用10 mU/mL促甲状腺激素（TSH）培养（TSH组）、用TSH和碘化钠培养（碘化钠组）或不用（对照组），再培养3天。在培养第8天，测量细胞内和细胞外环磷酸腺苷（cAMP）的量、细胞外cAMP和甲状腺球蛋白（TG）、过氧化物酶（PO）活性以及细胞数量。细胞内和细胞外cAMP的量相关性良好。TSH使cAMP、TG和PO的值升高至高于对照组的水平。随着添加到培养基中的碘化钠量增加，这些值降低。添加10⁻⁵ mol/L碘化钠仅降低cAMP的值。当添加10⁻⁴ mol/L碘化钠时，这三个水平低于TSH组，且cAMP的值几乎与对照组相等。在细胞数量方面，用TSH、TSH和碘化钠培养的细胞与不用TSH或碘化钠培养的细胞之间未发现差异。当用1 mmol/L二丁酰环磷腺苷钠盐或8-溴腺苷3',5'-环磷酸代替TSH培养甲状腺细胞时，10⁻⁴ mol/L碘化钠不会降低甲状腺球蛋白和过氧化物酶活性的值。这些结果表明，碘化钠仅在cAMP产生水平阻断TSH引发的细胞内信号转导。
