一种使用分离的青鳉黑素细胞对促黑素激素进行的灵敏生物测定法。

A sensitive bioassay for melanotropic hormones using isolated medaka melanophores.

作者信息

Negishi S, Kawazoe I, Kawauchi H

机构信息

Department of Biology, Keio University, Yokohama, Japan.

出版信息

Gen Comp Endocrinol. 1988 Apr;70(1):127-32. doi: 10.1016/0016-6480(88)90101-3.

DOI:10.1016/0016-6480(88)90101-3

PMID:2836261

Abstract

Melanophore-stimulating hormones (MSHs) from chum salmon cause pigment dispersion in isolated melanophores of medaka, a teleost. The in vitro medaka melanophore bioassay that responded to light with pigment dispersion and to the dark with pigment aggregation was utilized for measuring the activity of melanotropic hormones. alpha-MSH I was the most potent melanophore-dispersing agent tested. The minimal dose for the induction of pigment dispersion was 10(-15) M alpha-MSH I, 10(-13) M N-des-acetyl(Ac)-alpha-MSH, and 10(-11) M beta-MSH I, respectively. The melanosome-dispersing activity of beta-MSH I was enhanced about 40% by salmon N-acetyl-endorphin I (N-Ac-EP). The results suggest that N-Ac-EP may act as an enhancer for the activity of certain MSHs. The present bioassay provides a unique method for determining the biological activity of melanotropic peptides.

摘要

来自大麻哈鱼的促黑素细胞激素（MSHs）可使硬骨鱼青鳉分离出的黑素细胞发生色素扩散。利用体外青鳉黑素细胞生物测定法来测量促黑素激素的活性，该方法在光照下会使色素扩散，在黑暗中会使色素聚集。α-MSH I是所测试的最有效的黑素细胞分散剂。诱导色素扩散的最小剂量分别为10^(-15)M的α-MSH I、10^(-13)M的N-去乙酰基（Ac）-α-MSH和10^(-11)M的β-MSH I。鲑鱼N-乙酰基内啡肽I（N-Ac-EP）可使β-MSH I的黑素体分散活性增强约40%。结果表明，N-Ac-EP可能作为某些MSHs活性的增强剂。目前的生物测定法为确定促黑素肽的生物活性提供了一种独特的方法。