Castrucci A M, Hadley M E, Hruby V J
Gen Comp Endocrinol. 1987 Jun;66(3):374-80. doi: 10.1016/0016-6480(87)90247-4.
A teleost (the eel, Synbranchus marmoratus) skin bioassay for melanotropic peptides and other agonists is described. Unlike previous teleost assays that generally monitor or observe individual melanophores, this objective assay utilizes large intact pieces of skin and quantitative photoreflectance methods. Since melanosomes within most teleost melanophores are generally dispersed, the present assay provides a method for measuring the response of integumental melanophores to melanosome-aggregating agents such as MCH, a putative melanin-concentrating hormone. This bioassay is sensitive to MCH at a concentration as low as 10(-12) M. Because of the magnitude of this lightening response, four-point dose-response curves can be obtained. Skins lightened by MCH can then be used for bioassay of melanotropins or other melanosome-dispersing agents, such as beta-adrenoceptor agonists. This bioassay is unique in providing a method for determining the biological activities of melanotropic peptides with opposing actions.
本文描述了一种用于检测促黑素肽及其他激动剂的硬骨鱼（如鳗鲡，美洲鳗）皮肤生物测定法。与以往通常监测或观察单个黑素细胞的硬骨鱼测定法不同，这种客观的测定法使用大片完整皮肤和定量光反射法。由于大多数硬骨鱼黑素细胞内的黑素体通常是分散的，因此本测定法提供了一种测量体表黑素细胞对黑素体聚集剂（如一种假定的黑色素浓缩激素MCH）反应的方法。这种生物测定法对低至10（-12）M浓度的MCH敏感。由于这种变淡反应的程度，可以获得四点剂量反应曲线。然后，用MCH变淡的皮肤可用于促黑素或其他黑素体分散剂（如β-肾上腺素能受体激动剂）的生物测定。这种生物测定法的独特之处在于提供了一种确定具有相反作用的促黑素肽生物活性的方法。
