Min Min, Chen Xi-Bei, Wang Ping, Landeck Lilla, Chen Jia-Qi, Li Wei, Cai Sui-Qing, Zheng Min, Man Xiao-Yong
Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Ernst von Bergmann General Hospital, Teaching Hospital of Charité, Humboldt University, Potsdam, Germany.
PLoS One. 2017 Mar 31;12(3):e0174626. doi: 10.1371/journal.pone.0174626. eCollection 2017.
Keratin 24 (K24) is a new kind of keratin genes, which encodes a novel keratin protein, K24 that bears high similarity to the type I keratins and displays a unique expression profile. However, the role of K24 is incompletely understood. In our study, we investigated the localization of K24 within the epidermis and possible functions. Keratin 24 was found to be modestly overexpressed in senescent keratinocytes and was mainly restricted to the upper stratum spinosum of epidermis. The protein was required for terminal differentiation upon CaCl2-induced differentiation. In vitro results showed that increased K24 in keratinocytes dramatically changed the differentiation of primary keratinocytes. It also inhibited cell survival by G1/S phase cell cycle arrest and induced senescence, autophagy and apoptosis of keratinocytes. In addition, K24 activated PKCδ signal pathway involving in cellular survival. In summary, K24 may be suggested as a potential differentiation marker and anti-proliferative factor in the epidermis.
角蛋白24（K24）是一种新型角蛋白基因，它编码一种新型角蛋白K24，该蛋白与I型角蛋白具有高度相似性，并呈现出独特的表达谱。然而，K24的作用尚未完全明确。在我们的研究中，我们研究了K24在表皮中的定位及其可能的功能。研究发现，K24在衰老角质形成细胞中适度过表达，并且主要局限于表皮的棘层上层。在氯化钙诱导分化时，该蛋白是终末分化所必需的。体外实验结果表明，角质形成细胞中K24的增加显著改变了原代角质形成细胞的分化。它还通过G1/S期细胞周期阻滞抑制细胞存活，并诱导角质形成细胞衰老、自噬和凋亡。此外，K24激活了涉及细胞存活的PKCδ信号通路。总之，K24可能是表皮中一种潜在的分化标志物和抗增殖因子。
