Pickar Adrian, Xu Pei, Elson Andrew, Zengel James, Sauder Christian, Rubin Steve, He Biao
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.
Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, United States of America.
PLoS One. 2017 Mar 31;12(3):e0174444. doi: 10.1371/journal.pone.0174444. eCollection 2017.
Although mumps vaccines have been used for several decades, protective immune correlates have not been defined. Recently, mumps outbreaks have occurred in vaccinated populations. To better understand the causes of the outbreaks and to develop means to control outbreaks in mumps vaccine immunized populations, defining protective immune correlates will be critical. Unfortunately, no small animal model for assessing mumps immunity exists. In this study, we evaluated use of type I interferon (IFN) alpha/beta receptor knockout mice (IFN-α/βR-/-) for such a model. We found these mice to be susceptible to mumps virus administered intranasally and intracranially. Passive transfer of purified IgG from immunized mice protected naïve mice from mumps virus infection, confirming the role of antibody in protection and demonstrating the potential for this model to evaluate mumps immunity.
尽管腮腺炎疫苗已使用了数十年，但保护性免疫相关因素尚未明确。最近，在接种过疫苗的人群中发生了腮腺炎疫情。为了更好地理解疫情爆发的原因并开发控制腮腺炎疫苗免疫人群中疫情爆发的方法，确定保护性免疫相关因素至关重要。不幸的是，不存在用于评估腮腺炎免疫力的小动物模型。在本研究中，我们评估了使用I型干扰素（IFN）α/β受体敲除小鼠（IFN-α/βR-/-）作为这样一种模型。我们发现这些小鼠对经鼻内和颅内接种的腮腺炎病毒易感。将免疫小鼠的纯化IgG进行被动转移可保护未免疫小鼠免受腮腺炎病毒感染，证实了抗体在保护中的作用，并证明了该模型评估腮腺炎免疫力的潜力。
