Hiasa Y, Ohshima M, Kitahori Y, Konishi N, Shimoyama T, Sakaguchi Y, Hashimoto H, Minami S, Kato Y
First Department of Pathology, Nara Medical University.
Jpn J Cancer Res. 1988 Mar;79(3):314-9. doi: 10.1111/j.1349-7006.1988.tb01593.x.
The effects of erythrosine (Red 3), rose bengal B (Red 105) and thyroidectomy on the development of thyroid tumor were examined in male Wistar rats treated with N-bis(2-hydroxypropyl)nitrosamine (DHPN). Red 3 and Red 105 were used at 4% in the basal diet and were administered for 19 weeks from week 2 to 20. Thyroidectomy was performed by resection of the left lobe at week 4. Single injection of DHPN was performed intraperitoneally at 280 mg per 100 g body weight at the beginning of the experiment. Red 3 and Red 105 significantly promoted the development of thyroid tumors in thyroidectomized rats given DHPN, but had no significant effect in non-thyroidectomized rats. The incidence of thyroid tumors was 91% in rats with partial thyroidectomy, Red 3 and DHPN, 100% in rats with partial thyroidectomy, Red 105 and DHPN, and 64% in rats with partial thyroidectomy and DHPN. Serum TSH was 5.5 +/- 3.1 ng/ml in rats with partial thyroidectomy, Red 3 and DHPN, 2.1 +/- 2.2 ng/ml in rats with partial thyroidectomy, Red 105 and DHPN, and 1.5 +/- 0.5 ng/ml in rats with partial thyroidectomy and DHPN.
在接受N-双（2-羟丙基）亚硝胺（DHPN）处理的雄性Wistar大鼠中，研究了赤藓红（红色3号）、孟加拉玫瑰红B（红色105号）和甲状腺切除术对甲状腺肿瘤发生发展的影响。红色3号和红色105号以4%的比例添加到基础饲料中，从第2周持续给予19周直至第20周。在第4周通过切除左叶进行甲状腺切除术。在实验开始时，以每100克体重280毫克的剂量腹腔注射一次DHPN。红色3号和红色105号显著促进了接受DHPN处理的甲状腺切除大鼠甲状腺肿瘤的发生发展，但对未进行甲状腺切除的大鼠没有显著影响。部分甲状腺切除、红色3号和DHPN处理的大鼠甲状腺肿瘤发生率为91%，部分甲状腺切除、红色105号和DHPN处理的大鼠为100%，部分甲状腺切除和DHPN处理的大鼠为64%。部分甲状腺切除、红色3号和DHPN处理的大鼠血清促甲状腺激素（TSH）为5.5±3.1纳克/毫升，部分甲状腺切除、红色105号和DHPN处理的大鼠为2.1±2.2纳克/毫升，部分甲状腺切除和DHPN处理的大鼠为1.5±0.5纳克/毫升。
